| IED ID | IndEnz0002005767 |
| Enzyme Type ID | protease005767 |
| Protein Name |
Plasma kallikrein EC 3.4.21.34 Fletcher factor Kininogenin Plasma prekallikrein PKK Cleaved into: Plasma kallikrein heavy chain; Plasma kallikrein light chain |
| Gene Name | KLKB1 KLK3 |
| Organism | Homo sapiens (Human) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human) |
| Enzyme Sequence | MILFKQATYFISLFATVSCGCLTQLYENAFFRGGDVASMYTPNAQYCQMRCTFHPRCLLFSFLPASSINDMEKRFGCFLKDSVTGTLPKVHRTGAVSGHSLKQCGHQISACHRDIYKGVDMRGVNFNVSKVSSVEECQKRCTNNIRCQFFSYATQTFHKAEYRNNCLLKYSPGGTPTAIKVLSNVESGFSLKPCALSEIGCHMNIFQHLAFSDVDVARVLTPDAFVCRTICTYHPNCLFFTFYTNVWKIESQRNVCLLKTSESGTPSSSTPQENTISGYSLLTCKRTLPEPCHSKIYPGVDFGGEELNVTFVKGVNVCQETCTKMIRCQFFTYSLLPEDCKEEKCKCFLRLSMDGSPTRIAYGTQGSSGYSLRLCNTGDNSVCTTKTSTRIVGGTNSSWGEWPWQVSLQVKLTAQRHLCGGSLIGHQWVLTAAHCFDGLPLQDVWRIYSGILNLSDITKDTPFSQIKEIIIHQNYKVSEGNHDIALIKLQAPLNYTEFQKPICLPSKGDTSTIYTNCWVTGWGFSKEKGEIQNILQKVNIPLVTNEECQKRYQDYKITQRMVCAGYKEGGKDACKGDSGGPLVCKHNGMWRLVGITSWGEGCARREQPGVYTKVAEYMDWILEKTQSSDGKAQMQSPA |
| Enzyme Length | 638 |
| Uniprot Accession Number | P03952 |
| Absorption | |
| Active Site | ACT_SITE 434; /note=Charge relay system; ACT_SITE 483; /note=Charge relay system; ACT_SITE 578; /note=Charge relay system |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by SERPINA5. {ECO:0000269|PubMed:2844223}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Cleaves selectively Arg-|-Xaa and Lys-|-Xaa bonds, including Lys-|-Arg and Arg-|-Ser bonds in (human) kininogen to release bradykinin.; EC=3.4.21.34; |
| DNA Binding | |
| EC Number | 3.4.21.34 |
| Enzyme Function | FUNCTION: The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Beta strand (39); Chain (2); Disulfide bond (18); Domain (5); Glycosylation (5); Helix (18); Natural variant (13); Signal peptide (1); Turn (3) |
| Keywords | 3D-structure;Blood coagulation;Direct protein sequencing;Disease variant;Disulfide bond;Fibrinolysis;Glycoprotein;Hemostasis;Hydrolase;Inflammatory response;Protease;Reference proteome;Repeat;Secreted;Serine protease;Signal;Zymogen |
| Interact With | Q8TAB7; Q9UI10; O00746; C9J082; O14744; Q8TAS3; O00233; Q8IYM2; Q9UMY4; O43493-5; Q8NFB2; Q8N0U8 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..19 |
| Structure 3D | X-ray crystallography (13) |
| Cross Reference PDB | 2ANW; 2ANY; 4OGX; 4OGY; 5F8T; 5F8X; 5F8Z; 5TJX; 6I44; 6O1G; 6O1S; 6T7P; 7N7X; |
| Mapped Pubmed ID | 1066663; 11204562; 11830581; 11986212; 12123826; 12372819; 12773530; 12887060; 12944405; 1451784; 14691562; 14996700; 16199530; 16699514; 17318641; 17696780; 17922805; 18160576; 18413232; 18713009; 19342086; 19372141; 19423540; 19583818; 19620246; 20135073; 2026621; 20406964; 20438785; 20725119; 2176865; 2296585; 2365061; 23672780; 24552232; 24970892; 24977287; 25075649; 25324000; 25477429; 2563376; 2579948; 25891023; 26566265; 27130860; 27581227; 28124063; 28187111; 28197309; 28321; 29130992; 29186361; 29237166; 30394658; 3058116; 30591525; 30801944; 30817230; 30876891; 31473403; 32202057; 32551603; 33073460; 34436898; 3484703; 3756141; 3886654; 4054110; 4178758; 500690; 6203908; 6604055; 7391081; 7814636; 7826345; 8344943; 8529136; 874082; 8798678; 9226169; 9427705; |
| Motif | |
| Gene Encoded By | |
| Mass | 71,370 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.4.21.34; |