| IED ID | IndEnz0002005770 |
| Enzyme Type ID | protease005770 |
| Protein Name |
Josephin-1 EC 3.4.19.12 Josephin domain-containing protein 1 |
| Gene Name | Josd1 |
| Organism | Rattus norvegicus (Rat) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Rattus Rattus norvegicus (Rat) |
| Enzyme Sequence | MSCMPWKGDKAKSESLELPQAAPPQIYHEKQRRELCALHALNNVFQDSNAFTRETLQEIFQRLSPNTVVTPHKKSMLGNGNYDVNVIMAALQTKGYEAVWWDKRRDVGVIALANVMGFIMNLPSSLCWGPLKLPLKRQHWICVREVGGAYYNLDSKLKMPEWIGGESELRKFLKYHLRGKNCELLLVVPEEVEAHQSWRADV |
| Enzyme Length | 202 |
| Uniprot Accession Number | Q5BJY4 |
| Absorption | |
| Active Site | ACT_SITE 36; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU00331; ACT_SITE 139; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU00331 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Deubiquitinates monoubiquitinated probes (in vitro). When ubiquitinated, cleaves 'Lys-63'-linked and 'Lys-48'-linked poly-ubiquitin chains (in vitro), hence may act as a deubiquitinating enzyme. May increase macropinocytosis and suppress clathrin- and caveolae-mediated endocytosis. May enhance membrane dynamics and cell motility independently of its catalytic activity (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Domain (1); Modified residue (1); Region (1) |
| Keywords | Cell membrane;Cytoplasm;Hydrolase;Membrane;Phosphoprotein;Protease;Reference proteome;Ubl conjugation;Ubl conjugation pathway |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}. Cytoplasm {ECO:0000250}. Note=Ubiquitination increases localization the plasma membrane. In the cytosol, the unubiquitinated form may be associated with the cytoskeleton via ACTB-binding. {ECO:0000250}. |
| Modified Residue | MOD_RES 15; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:Q15040 |
| Post Translational Modification | PTM: Monoubiquitinated. Ubiquitination activates deubiquitination activity in vitro. {ECO:0000250}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 23,178 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |