| IED ID | IndEnz0002005774 |
| Enzyme Type ID | protease005774 |
| Protein Name |
Josephin-2 EC 3.4.19.12 Josephin domain-containing protein 2 |
| Gene Name | JOSD2 SBBI54 |
| Organism | Homo sapiens (Human) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human) |
| Enzyme Sequence | MSQAPGAQPSPPTVYHERQRLELCAVHALNNVLQQQLFSQEAADEICKRLAPDSRLNPHRSLLGTGNYDVNVIMAALQGLGLAAVWWDRRRPLSQLALPQVLGLILNLPSPVSLGLLSLPLRRRHWVALRQVDGVYYNLDSKLRAPEALGDEDGVRAFLAAALAQGLCEVLLVVTKEVEEKGSWLRTD |
| Enzyme Length | 188 |
| Uniprot Accession Number | Q8TAC2 |
| Absorption | |
| Active Site | ACT_SITE 24; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU00331; ACT_SITE 125; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU00331 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; Evidence={ECO:0000269|PubMed:17696782, ECO:0000269|PubMed:21118805}; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Cleaves 'Lys-63'-linked poly-ubiquitin chains, and with lesser efficiency 'Lys-48'-linked poly-ubiquitin chains (in vitro). May act as a deubiquitinating enzyme. {ECO:0000269|PubMed:17696782, ECO:0000269|PubMed:21118805, ECO:0000269|PubMed:23625928}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Alternative sequence (1); Beta strand (7); Chain (1); Domain (1); Helix (7); Mutagenesis (1); Turn (2) |
| Keywords | 3D-structure;Alternative splicing;Cytoplasm;Hydrolase;Protease;Reference proteome;Ubl conjugation pathway |
| Interact With | O43865; Q96DZ9-2; Q8NI60; Q9Y680; P21741; P08247; P0DI81-3; P49638; O95070 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000269|PubMed:23625928}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 6PGV; |
| Mapped Pubmed ID | 33082514; |
| Motif | |
| Gene Encoded By | |
| Mass | 20,756 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |