| IED ID | IndEnz0002005790 |
| Enzyme Type ID | protease005790 |
| Protein Name |
CD177 antigen Human neutrophil alloantigen 2a HNA-2a NB1 glycoprotein NB1 GP Polycythemia rubra vera protein 1 PRV-1 CD antigen CD177 |
| Gene Name | CD177 NB1 PRV1 UNQ595/PRO1181 |
| Organism | Homo sapiens (Human) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human) |
| Enzyme Sequence | MSAVLLLALLGFILPLPGVQALLCQFGTVQHVWKVSDLPRQWTPKNTSCDSGLGCQDTLMLIESGPQVSLVLSKGCTEAKDQEPRVTEHRMGPGLSLISYTFVCRQEDFCNNLVNSLPLWAPQPPADPGSLRCPVCLSMEGCLEGTTEEICPKGTTHCYDGLLRLRGGGIFSNLRVQGCMPQPGCNLLNGTQEIGPVGMTENCNRKDFLTCHRGTTIMTHGNLAQEPTDWTTSNTEMCEVGQVCQETLLLLDVGLTSTLVGTKGCSTVGAQNSQKTTIHSAPPGVLVASYTHFCSSDLCNSASSSSVLLNSLPPQAAPVPGDRQCPTCVQPLGTCSSGSPRMTCPRGATHCYDGYIHLSGGGLSTKMSIQGCVAQPSSFLLNHTRQIGIFSAREKRDVQPPASQHEGGGAEGLESLTWGVGLALAPALWWGVVCPSC |
| Enzyme Length | 437 |
| Uniprot Accession Number | Q8N6Q3 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: In association with beta-2 integrin heterodimer ITGAM/CD11b and ITGB2/CD18, mediates activation of TNF-alpha primed neutrophils including degranulation and superoxide production (PubMed:21193407). In addition, by preventing beta-2 integrin internalization and attenuating chemokine signaling favors adhesion over migration (PubMed:28807980). Heterophilic interaction with PECAM1 on endothelial cells plays a role in neutrophil transendothelial migration in vitro (PubMed:17580308). However, appears to be dispensable for neutrophil recruitment caused by bacterial infection in vivo (PubMed:23461681). Acts as a receptor for the mature form of protease PRTN3 allowing its display at the cell surface of neutrophils (PubMed:17244676, PubMed:18462208). By displaying PRTN3 at the neutrophil cell surface, may play a role in enhancing endothelial cell junctional integrity and thus vascular integrity during neutrophil diapedesis (PubMed:23202369). {ECO:0000269|PubMed:17244676, ECO:0000269|PubMed:17580308, ECO:0000269|PubMed:18462208, ECO:0000269|PubMed:21193407, ECO:0000269|PubMed:23202369, ECO:0000269|PubMed:23461681, ECO:0000269|PubMed:28807980}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Alternative sequence (4); Chain (1); Domain (2); Glycosylation (1); Lipidation (1); Natural variant (9); Propeptide (1); Sequence conflict (4); Signal peptide (1) |
| Keywords | Alternative splicing;Cell adhesion;Cell membrane;Cell projection;Direct protein sequencing;GPI-anchor;Glycoprotein;Immunity;Innate immunity;Lipoprotein;Membrane;Reference proteome;Repeat;Secreted;Signal |
| Interact With | Q9Y2W7 |
| Induction | INDUCTION: By CSF3/G-CSF in resting granulocytes (PubMed:10753836). Induced during CSF3/G-CSF-mediated neutrophil differentiation (PubMed:17244676, PubMed:18462208). Induced during pregnancy (PubMed:12675722). Induced in patients with polycythemia vera (PV) and with essential thrombocythemia (ET) (PubMed:10753836, PubMed:12377969). {ECO:0000269|PubMed:10753836, ECO:0000269|PubMed:12377969, ECO:0000269|PubMed:12675722, ECO:0000269|PubMed:17244676, ECO:0000269|PubMed:18462208}. |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:12239154, ECO:0000269|PubMed:12675722, ECO:0000269|PubMed:17244676, ECO:0000269|PubMed:17580308, ECO:0000269|PubMed:18462208, ECO:0000269|PubMed:21193407, ECO:0000269|PubMed:24926686, ECO:0000269|PubMed:27227454, ECO:0000269|PubMed:28240246, ECO:0000269|PubMed:28807980}; Lipid-anchor, GPI-anchor {ECO:0000269|PubMed:12239154, ECO:0000269|PubMed:17244676}. Membrane raft {ECO:0000269|PubMed:21193407}; Lipid-anchor, GPI-like-anchor {ECO:0000269|PubMed:12239154, ECO:0000269|PubMed:17244676}. Secreted {ECO:0000269|PubMed:12239154, ECO:0000269|PubMed:18462208}. Cytoplasmic granule membrane {ECO:0000269|PubMed:18462208}. Cell projection, lamellipodium {ECO:0000269|PubMed:28807980}. Note=Cell surface expression on neutrophils is increased upon TNF-alpha, fMLP or CXCL8/IL8-mediated stimulation (PubMed:17244676, PubMed:17580308). In neutrophils, stored predominantly in secondary and tertiary granules (PubMed:18462208). Can also be shedded from the cell membrane (PubMed:12239154, PubMed:18462208). Localizes to lamellar protrusions in spreading neutrophils (PubMed:28807980). {ECO:0000269|PubMed:12239154, ECO:0000269|PubMed:17244676, ECO:0000269|PubMed:17580308, ECO:0000269|PubMed:18462208, ECO:0000269|PubMed:28807980}. |
| Modified Residue | |
| Post Translational Modification | PTM: N-glycosylated. {ECO:0000269|PubMed:11465086, ECO:0000269|PubMed:12239154}.; PTM: A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding). {ECO:0000269|PubMed:12239154, ECO:0000269|PubMed:18462208}. |
| Signal Peptide | SIGNAL 1..21; /evidence=ECO:0000269|PubMed:15340161 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11035113; 12591276; 12617169; 12756017; 12901344; 14701686; 15043575; 15572213; 15951300; 15985544; 16189514; 16502591; 16682284; 17116209; 17976520; 17980909; 18156711; 18854317; 19185066; 19695014; 19999391; 20194726; 22266279; 22300941; 22690867; 23650620; 23899160; 24124144; 25416956; 26024230; 26829180; 27568821; 28559244; 30201498; 30377339; 30674924; 30943984; 31777111; 32042113; 32341565; 32531826; 33302660; 33734454; 34599187; 34879110; |
| Motif | |
| Gene Encoded By | |
| Mass | 46,363 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |