| IED ID | IndEnz0002005795 |
| Enzyme Type ID | protease005795 |
| Protein Name |
Pesticidal crystal protein Cry6Aa Crystaline entomocidal protoxin Crystal protein Insecticidal delta-endotoxin CryVIA a Fragment |
| Gene Name | cry6Aa cryVIa cryVIA(a) |
| Organism | Bacillus thuringiensis |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus cereus group Bacillus thuringiensis |
| Enzyme Sequence | MIIDSKTTLPRHSLIHTIKLNSNKKYGPGDMTNGNQFIISKQEWATIGAYIQTGLGLPVNEQQLRTHVNLSQDISIPSDFSQLYDVYCSDKTSAEWWNKNLYPLIIKSANDIASYGFKVAGDPSIKKDGYFKKLQDELDNIVDNNSDDDAIAKAIKDFKARCGILIKEAKQYEEAAKNIVTSLDQFLHGDQKKLEGVINIQKRLKEVQTALNQAHGESSPAHKELLEKVKNLKTTLERTIKAEQDLEKKVEYSFLLGPLLGFVVYEILENTAVQHIKNQIDEIKKQLDSAQHDLDRDVKIIGMLNSINTDIDNLYSQGQEAIKVFQKLQGIWATIGAQIENLRTTSLQEVQDSDDADEIQIELEDASDAWLVVAQEARDFTLNAYSTNSRQNLPINVISDSCNCSTTNMTSNQYSNPTTNMTSNQYMISHEYTSLPNNFMLSRNSNLEYKCPENNFMIYWYNNSDWYNNSDWYNN |
| Enzyme Length | 475 |
| Uniprot Accession Number | Q45757 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Pore-forming toxin with nematicidal activity (PubMed:26795495, PubMed:27576487). In infected C.elegans, induces an increase in intracellular Ca(2+) resulting in necrosis of host intestinal cells (PubMed:26795495). Also, induces the expression of aspartic protease asp-1 (PubMed:26795495). {ECO:0000269|PubMed:26795495, ECO:0000269|PubMed:27576487}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Beta strand (4); Chain (1); Disulfide bond (2); Helix (18); Mutagenesis (1); Non-terminal residue (1); Region (1); Turn (3) |
| Keywords | 3D-structure;Disulfide bond;Host cell membrane;Host membrane;Membrane;Secreted;Sporulation;Toxin;Virulence |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000305}. Host cell membrane {ECO:0000305|PubMed:27576487}. Note=Probably secreted as a soluble protein that then inserts into the host membrane and forms pores. {ECO:0000305|PubMed:27576487}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (3) |
| Cross Reference PDB | 5GHE; 5KUC; 5KUD; |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 54,075 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |