| IED ID | IndEnz0002005844 |
| Enzyme Type ID | protease005844 |
| Protein Name |
Carboxyl-terminal-processing peptidase 1, chloroplastic EC 3.4.21.102 D1 C-terminal processing protease 1 Photosystem II D1 protein processing peptidase 1 |
| Gene Name | CTPA1 At5g46390 MPL12.19 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MRLLLPFSSPLSATSSPSTPQFIPELPPPSQFDYSGLTKILKKSVIGTLTGALSLTLVFSSPISSVAATNDPYLSVNPPSSSFESSLNHFDSAPEDCPNEEEADTEIQDDDIEPQLVTNEGIVEEAWEIVNGAFLDTRSHSWTPETWQKQKDDILASPIKSRSKAHEVIKNMLASLGDQYTRFLSPDEFSRMSKYDITGIGINLREVSDGGGNVKLKVLGLVLDSAADIAGVKQGDEILAVNGMDVSGKSSFEVSSLLQGPSKTFVVLKVKHGKCGPVKSLKIQRQVNAQTPVSYRLEKVDNGTVSVGYIRLKEFNALARKDLVIAMKRLLDKGASYFVMDLRDNLGGLVQAGIETAKLFLDEGDTVIYTAGRDPEAQKTVVSDKKPLITAPLIVMVNNRTASASEIVASALHDNCKAVLVGERTYGKGLIQSVYELRDGSGVVVTIGKYVTPNHMDINGGGIEPDFRNLPAWDEVKERLSKCSILQQS |
| Enzyme Length | 489 |
| Uniprot Accession Number | F4KHG6 |
| Absorption | |
| Active Site | ACT_SITE 403; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 428; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=The enzyme shows specific recognition of a C-terminal tripeptide, Xaa-Yaa-Zaa, in which Xaa is preferably Ala or Leu, Yaa is preferably Ala or Tyr, and Zaa is preferably Ala, but then cleaves at a variable distance from the C-terminus. A typical cleavage is -Ala-Ala-|-Arg-Ala-Ala-Lys-Glu-Asn-Tyr-Ala-Leu-Ala-Ala.; EC=3.4.21.102; |
| DNA Binding | |
| EC Number | 3.4.21.102 |
| Enzyme Function | FUNCTION: Protease involved in the C-terminal processing of the chloroplastic D1 protein of photosystem II. This proteolytic processing is necessary to allow the light-driven assembly of the tetranuclear manganese cluster, which is responsible for photosynthetic water oxidation. {ECO:0000250|UniProtKB:O04073}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Alternative sequence (2); Chain (1); Domain (1); Erroneous gene model prediction (1); Region (1); Transit peptide (2) |
| Keywords | Alternative splicing;Chloroplast;Direct protein sequencing;Hydrolase;Plastid;Protease;Reference proteome;Serine protease;Thylakoid;Transit peptide |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid lumen {ECO:0000269|PubMed:11719511}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 12185496; 14576160; 20049866; 24023856; |
| Motif | |
| Gene Encoded By | |
| Mass | 53,033 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |