| IED ID | IndEnz0002005883 |
| Enzyme Type ID | protease005883 |
| Protein Name |
Chitin-binding protein CbpD Protease LasD |
| Gene Name | cbpD lasD PA0852 |
| Organism | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Pseudomonadales Pseudomonadaceae Pseudomonas Pseudomonas aeruginosa group Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) |
| Enzyme Sequence | MKHYSATLALLPLTLALFLPQAAHAHGSMETPPSRVYGCFLEGPENPKSAACKAAVAAGGTQALYDWNGVNQGNANGNHQAVVPDGQLCGAGKALFKGLNLARSDWPSTAIAPDASGNFQFVYKASAPHATRYFDFYITKDGYNPEKPLAWSDLEPAPFCSITSVKLENGTYRMNCPLPQGKTGKHVIYNVWQRSDSPEAFYACIDVSFSGAVANPWQALGNLRAQQDLPAGATVTLRLFDAQGRDAQRHSLTLAQGANGAKQWPLALAQKVNQDSTLVNIGVLDAYGAVSPVASSQDNQVYVRQAGYRFQVDIELPVEGGGEQPGGDGKVDFDYPQGLQQYDAGTVVRGADGKRYQCKPYPNSGWCKGWDLYYAPGKGMAWQDAWTLL |
| Enzyme Length | 389 |
| Uniprot Accession Number | Q9I589 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Binds chitin but does not hydrolyze it, has no detectable protease or staphylolytic activity. {ECO:0000269|PubMed:10671445}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Domain (1); Sequence conflict (1); Signal peptide (1) |
| Keywords | Chitin-binding;Direct protein sequencing;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:10671445, ECO:0000269|PubMed:9642203}. Note=Secreted in an Xcp-dependent fashion (a type II secretion pathway). {ECO:0000269|PubMed:10671445, ECO:0000269|PubMed:9642203}. |
| Modified Residue | |
| Post Translational Modification | PTM: Can be detected in the extracellular supernatant as a 43 kDa protein and a 23 kDa protein, both proteins have the same N-terminus (PubMed:9642203, PubMed:10671445). Only the larger protein binds chitin, which may protect it from further processing and/or degradation by elastase (lasB) (PubMed:10671445, PubMed:9642203). It is not clear whether the short form is functional or a degradation product. {ECO:0000269|PubMed:10671445, ECO:0000269|PubMed:9642203, ECO:0000305}. |
| Signal Peptide | SIGNAL 1..25; /evidence="ECO:0000269|PubMed:7565088, ECO:0000269|PubMed:9642203" |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 41,917 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |