| IED ID | IndEnz0002005937 |
| Enzyme Type ID | protease005937 |
| Protein Name |
L-shaped tail fiber protein pb1 LTF EC 3.4.21.- Tail protein pb1 |
| Gene Name | ltf T5.133 T5p131 |
| Organism | Escherichia phage T5 (Enterobacteria phage T5) |
| Taxonomic Lineage | Viruses Duplodnaviria Heunggongvirae Uroviricota Caudoviricetes Caudovirales Demerecviridae Markadamsvirinae Tequintavirus Escherichia phage T5 (Enterobacteria phage T5) |
| Enzyme Sequence | MAITKIILQQMVTMDQNSITASKYPKYTVVLSNSISSITAADVTSAIESSKASGPAAKQSEINAKQSELNAKDSENEAEISATSSQQSATQSASSATASANSAKAAKTSETNANNSKNAAKTSETNAASSASSASSFATAAENSARAAKTSETNAGNSAQAADASKTAAANSATAAKTSETNAKKSETAAKTSETNAKTSENKAKEYLDMASELVSPVTQYDWPVGTNNNSVYVKIAKLTDPGAVSCHLTLMITNGGNYGSSYGNIDFVEISARGLNDARGVTSENITKFLSVRRLGSPNLAWDNQLRYGLVEGDGYFEVWCYQRAFIKETRVAVLAQTGRTELYIPEGFVSQDTQPSGFIESLAARIYDQVNKPTKADLGLENAMLVGAFGLGGNGLSYSSVQSNVDLINKLKANGGQYWRAARESGANVDINDHGSGFYSHCGDTHAAINVQYNTGIVKVLATTDRNLASDIVYANTLYGTANKPSKSDVGLGNVTNDAQVKKAGDVMSGDLDIRKETPSIRLKSTQGNAHLWFMNNDGGERGVIWSPPNNGSLGEIHIRAKTSDGTSTGDFIVRHDGRIEAKDAKISYKISSRTAEFSNDDTNTAATNLRVSGKQHTPIMLVRDSDSNVSVGFKLNNMNAKLLGIDIDGDLAFGENPDHKQNSKIVTRKMMDAGFSVAGLMDFTNGFAGPWEAKNISDQELDLNSLMIKKSDPGSIRVYQCVSAGGGNNITNKPSGIGGNFILYVESIRKVGDTDFTNRQRLFGTDLNREFTRYCSNGTWSAWRESVVSGMNQDVSVKSMSVSGRLSGNELSVGGAGVLNGNLGVGGGATSKMPSSDKGIVIGRGSIVREGGEGRLILSSSGGTDRLLQLRPAGATSLDNQVEISCTSASAGDTKISFGQGAAIRCNNAGSPIISAKAGQMIYFRPNGDGISEGQMILSPNGDLVVKGGVNSKEIDVTASQSLPLKETTATTGIGVNFIGDSATECSFGIENTAGGSAVFHNYTRGASNSVTKNNQLLGGYGSRPWLGSTYTEHSNAALHFLGAGDTSATNHGGWIRLLVTPKGKTISDRVPAFRLSDNGDLWLVPDGAMHSDLGLVRSIETLNAAVPRFNAPSIQDGRGLKIVAPQAPEIDLIAPRGSGASAPAIRAMWCDGSLADTTRYIGATQPGSTFYIGASGHDGEKFDSMRGSVAIKSAGGWGPTSTPTQVVLETCESGSISRLPRWGVDHNGTLMPMADNRYNLGWGSGRVKQVYAVNGTINTSDARLKNDVRAMSDPETEAAKAIAKEIGFWTWKEQADMNDIREHCGLTVQRAIEIMESFGLDPFKYGFICYDKWDEHTVVSEYGPANEDGTENPIYKTIPAGDHYSFRLEELNLFIAKGFEARLSAIEDKLGM |
| Enzyme Length | 1396 |
| Uniprot Accession Number | P13390 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Assembles together with p132 to form the three L-shaped long tail fibers and probably the collar structure at the junction between the tail tube and the conical tail tip (PubMed:24198424). The three L-shaped long tail fibers recognize the host lipopolysaccharides that serve as adhesion receptor for virus entry (PubMed:7045389). Each fiber consists of a thin proximal rod of about 30 nm connected by a hinge to a thicker distal part of about 47 nm (PubMed:24198424). {ECO:0000269|PubMed:24198424, ECO:0000269|PubMed:7045389}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Beta strand (26); Chain (1); Coiled coil (2); Compositional bias (3); Domain (1); Helix (10); Propeptide (1); Region (2); Sequence conflict (7); Site (1); Turn (1) |
| Keywords | 3D-structure;Coiled coil;Host-virus interaction;Hydrolase;Late protein;Protease;Reference proteome;Serine protease;Viral attachment to host adhesion receptor;Viral attachment to host cell;Viral tail fiber protein;Viral tail protein;Virion;Virus entry into host cell |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:24198424}. Note=Part of the tail long fibers. {ECO:0000269|PubMed:24198424}. |
| Modified Residue | |
| Post Translational Modification | PTM: The cleaved C-terminus functions as an intramolecular chaperone and is removed by an autoproteolytic process after correct trimerization and folding. {ECO:0000269|PubMed:24316831, ECO:0000269|PubMed:26670244}. |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (3) |
| Cross Reference PDB | 4UW7; 4UW8; 5AQ5; |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 147,962 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |