| IED ID | IndEnz0002006036 |
| Enzyme Type ID | protease006036 |
| Protein Name |
Probable zinc metalloprotease EGY1, chloroplastic EC 3.4.24.- Protein AMMONIUM OVERLY SENSITIVE 1 Protein ETHYLENE-DEPENDENT GRAVITROPISM-DEFICIENT AND YELLOW-GREEN 1 AtEGY1 |
| Gene Name | EGY1 AMOS1 At5g35220 T25C13.100 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MGTLTSVAFAAAVNIRFRSFHRENIKTTITTLPKWQKRLCFSSTEDSHRFRIAKCLGNDENSNRDDSIGENGETHKSSVVKTATFEEEDEETSKSSSTTSSSNEFGSDKTSMPSTIDPTYSSFQIDSFKLMELLGPEKVDPADVKLIKDKLFGYSTFWVTKEEPFGDLGEGILFLGNLRGKKEDVFAKLQRKLVEVASDKYNLFMIEEPNSEGPDPRGGARVSFGLLRKEVSEPGPTTLWQYVIALILFLLTIGSSVELGIASQINRLPPEVVKYFTDPNAVEPPDMELLYPFVDAALPLAYGVLGILLFHELGHFLAAVPKKVKLSIPYFIPNITLGSFGAITQFKSILPDRSTKVDISLAGPFAGAALSVSMFAVGLFLSTEPDAANDLVQVPSMLFQGSLLLGLISRATLGYAALHAATVSIHPLVIAGWCGLTTTAFNMLPVGCLDGGRAVQGAFGKNALVTFGLSTYVMLGLRVLGGPLALPWGLYVLICQRTPEKPCLNDVTEVGTWRKALVGIALILVVLTLLPVWDELAEEVGIGLVTTF |
| Enzyme Length | 548 |
| Uniprot Accession Number | Q949Y5 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.24.- |
| Enzyme Function | FUNCTION: Membrane-associated and ATP-independent metalloprotease required for development of both thylakoid grana and well-organized lamellae in chloroplast. Required for the accumulation of chlorophyll and chlorophyll a/b binding (CAB) proteins (from both PS I and PS II) in chloroplast membranes, and for grana formation and normal chloroplast development. Involved in the regulation of nuclear gene expression in response to ammonium stress and interacts with ABA signaling. Carries out beta-casein degradation in an ATP-independent manner in vitro. {ECO:0000269|PubMed:15659096, ECO:0000269|PubMed:18097640, ECO:0000269|PubMed:23064408}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Compositional bias (2); Erroneous gene model prediction (1); Region (1); Transit peptide (1); Transmembrane (8) |
| Keywords | Chloroplast;Developmental protein;Hydrolase;Membrane;Metalloprotease;Plastid;Protease;Reference proteome;Transit peptide;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | INDUCTION: By ethylene. Down-regulated by dark. {ECO:0000269|PubMed:15659096}. |
| Subcellular Location | SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}. Note=Localizes in endodermal and cortex plastids. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11152613; 12912986; 14576160; 18431481; 18650403; 21139083; 22579628; 22623517; 27516532; 28510790; 31836664; |
| Motif | |
| Gene Encoded By | |
| Mass | 59,501 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |