| IED ID | IndEnz0002006050 |
| Enzyme Type ID | protease006050 |
| Protein Name |
Gamma-D-glutamyl-L-diamino acid endopeptidase 1 EC 3.4.19.11 Endopeptidase I Gamma-D-glutamyl-L-diamino acid endopeptidase I Gamma-D-glutamyl-meso-diaminopimelate peptidase I |
| Gene Name | |
| Organism | Lysinibacillus sphaericus (Bacillus sphaericus) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Bacillaceae Lysinibacillus Lysinibacillus sphaericus (Bacillus sphaericus) |
| Enzyme Sequence | MDILIRPGDSLWYFSDLFKIPLQLLLDSNRNINPQLLQVGQRIQIPGYVTTSYTITQGDSLWQIAQNKNLPLNAILLVNPEIQPSRLHIGQTIQVPQRLTWRLVNGQQNYDYSMMMNDIKKLQTAYPFLQGTPIGNSVLAQPIPEILIGNGSKRIHYKASFHANEWITTPIIMTFLNDYLLALTNQTTIRGLSMGPLYNQTTLSLVPMVNPDGVNLVINGPPANEALKNKLIAWNHNSQNFSGWKANINGVDLNDQFPAKWELENARNPQTPGPRDYGGEAPLTQPEAIAMADLTRSRNFAWVLAFHTQGRVIYWGFENLEPPESQTMVEEFSRVSGYEPIQSANSYAGYKDWFIQDWRRPGFTVELGSGTNPLPISEFDTIYQEALGIFLAGLYL |
| Enzyme Length | 396 |
| Uniprot Accession Number | Q03415 |
| Absorption | |
| Active Site | ACT_SITE 347; /note=Proton donor; /evidence=ECO:0000250; ACT_SITE 366; /note=Nucleophile; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | BINDING 255; /note=Substrate; /evidence=ECO:0000250 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of gamma-D-glutamyl bonds to the L-terminus (position 7) of meso-diaminopimelic acid (meso-A(2)pm) in 7-(L-Ala-gamma-D-Glu)-meso-A(2)pm and 7-(L-Ala-gamma-D-Glu)-7-(D-Ala)-meso-A(2)pm. It is required that the D-terminal amino and carboxy groups of meso-A(2)pm are unsubstituted.; EC=3.4.19.11; |
| DNA Binding | |
| EC Number | 3.4.19.11 |
| Enzyme Function | FUNCTION: An endopeptidase which hydrolyzes the gamma-D-Glu-(L)meso-diaminopimelic acid bond of L-Ala-gamma-D-Glu-(L)meso-diaminopimelic acid and L-Ala-gamma-D-Glu-(L)meso-diaminopimelic acid(L)-D-Ala peptides. It is active on spore cortex peptidoglycan. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Binding site (1); Chain (1); Domain (2); Metal binding (3); Region (1) |
| Keywords | Cell wall biogenesis/degradation;Direct protein sequencing;Hydrolase;Metal-binding;Metalloprotease;Protease;Repeat;Sporulation;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 44,724 |
| Kinetics | |
| Metal Binding | METAL 162; /note=Zinc; /evidence=ECO:0000250; METAL 165; /note=Zinc; /evidence=ECO:0000250; METAL 307; /note=Zinc; /evidence=ECO:0000250 |
| Rhea ID | |
| Cross Reference Brenda |