| IED ID | IndEnz0002006099 |
| Enzyme Type ID | protease006099 |
| Protein Name |
Macrophage metalloelastase MME EC 3.4.24.65 Matrix metalloproteinase-12 MMP-12 |
| Gene Name | MMP12 |
| Organism | Oryctolagus cuniculus (Rabbit) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Lagomorpha Leporidae (rabbits and hares) Oryctolagus Oryctolagus cuniculus (Rabbit) |
| Enzyme Sequence | MKFLLLILTLWVTSSGADPLKENDMLFAENYLENFYGLKVERIPMTKMKTNRNFIEEKVQEMQQFLGLNVTGQLDTSTLEMMHKPRCGVPDVYHFKTMPGRPVWRKHYITYRIKNYTPDMKREDVEYAIQKAFQVWSDVTPLKFRKITTGKADIMILFASGAHGDYGAFDGRGGVIAHAFGPGPGIGGDTHFDEDEIWSKSYKGTNLFLVAVHELGHALGLDHSNDPKAIMFPTYGYIDLNTFHLSADDIRGIQSLYGGPEQHQPMPKPDNPEPTACDHNLKFDAVTTVGNKIFFFKDSFFWWKIPKSSTTSVRLISSLWPTLPSGIEAAYEIGDRHQVFLFKGDKFWLISHLRLQPNYPKSIHSLGFPDFVKKIDAAVFNPSLRKTYFFVDNLYWRYDERREVMDAGYPKLITKHFPGIGPKIDAVFYFQRYYYFFQGPNQLEYDTFSSRVTKKLKSNSWFDC |
| Enzyme Length | 464 |
| Uniprot Accession Number | P79227 |
| Absorption | |
| Active Site | ACT_SITE 214; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of soluble and insoluble elastin. Specific cleavages are also produced at 14-Ala-|-Leu-15 and 16-Tyr-|-Leu-17 in the B chain of insulin.; EC=3.4.24.65; |
| DNA Binding | |
| EC Number | 3.4.24.65 |
| Enzyme Function | FUNCTION: May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3 (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Disulfide bond (1); Glycosylation (1); Metal binding (25); Motif (1); Propeptide (1); Repeat (4); Signal peptide (1) |
| Keywords | Calcium;Disulfide bond;Extracellular matrix;Glycoprotein;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Repeat;Secreted;Signal;Zinc;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..17; /evidence=ECO:0000305 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 85..92; /note=Cysteine switch; /evidence=ECO:0000250 |
| Gene Encoded By | |
| Mass | 53,584 |
| Kinetics | |
| Metal Binding | METAL 87; /note=Zinc 2; in inhibited form; /evidence=ECO:0000250; METAL 119; /note=Calcium 1; /evidence=ECO:0000250; METAL 153; /note=Calcium 2; /evidence=ECO:0000250; METAL 163; /note=Zinc 1; /evidence=ECO:0000250; METAL 165; /note=Zinc 1; /evidence=ECO:0000250; METAL 170; /note=Calcium 3; /evidence=ECO:0000250; METAL 171; /note=Calcium 3; via carbonyl oxygen; /evidence=ECO:0000250; METAL 173; /note=Calcium 3; via carbonyl oxygen; /evidence=ECO:0000250; METAL 175; /note=Calcium 3; via carbonyl oxygen; /evidence=ECO:0000250; METAL 178; /note=Zinc 1; /evidence=ECO:0000250; METAL 185; /note=Calcium 2; via carbonyl oxygen; /evidence=ECO:0000250; METAL 187; /note=Calcium 2; via carbonyl oxygen; /evidence=ECO:0000250; METAL 189; /note=Calcium 2; /evidence=ECO:0000250; METAL 191; /note=Zinc 1; /evidence=ECO:0000250; METAL 193; /note=Calcium 3; /evidence=ECO:0000250; METAL 194; /note=Calcium 1; /evidence=ECO:0000250; METAL 196; /note=Calcium 1; /evidence=ECO:0000250; METAL 196; /note=Calcium 3; /evidence=ECO:0000250; METAL 213; /note=Zinc 2; catalytic; /evidence=ECO:0000250; METAL 217; /note=Zinc 2; catalytic; /evidence=ECO:0000250; METAL 223; /note=Zinc 2; catalytic; /evidence=ECO:0000250; METAL 284; /note=Calcium 4; via carbonyl oxygen; /evidence=ECO:0000250; METAL 328; /note=Calcium 4; via carbonyl oxygen; /evidence=ECO:0000250; METAL 376; /note=Calcium 4; via carbonyl oxygen; /evidence=ECO:0000250; METAL 425; /note=Calcium 4; via carbonyl oxygen; /evidence=ECO:0000250 |
| Rhea ID | |
| Cross Reference Brenda |