| IED ID | IndEnz0002006100 |
| Enzyme Type ID | protease006100 |
| Protein Name |
Macrophage metalloelastase MME EC 3.4.24.65 Matrix metalloproteinase-12 MMP-12 |
| Gene Name | Mmp12 Mmel |
| Organism | Rattus norvegicus (Rat) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Rattus Rattus norvegicus (Rat) |
| Enzyme Sequence | MKFLLVLVLLVSLQVSACGAAPMNESEFAEWYLSRFFDYQGDRIPMTKTKTNRNLLEEKLQEMQQFFGLEVTGQLDTSTLKIMHTSRCGVPDVQHLRAVPQRSRWMKRYLTYRIYNYTPDMKRADVDYIFQKAFQVWSDVTPLRFRKIHKGEADITILFAFGDHGDFYDFDGKGGTLAHAFYPGPGIQGDAHFDEAETWTKSFQGTNLFLVAVHELGHSLGLRHSNNPKSIMYPTYRYLHPNTFRLSADDIHSIQSLYGAPVKNPSLTNPGSPPSTVCHQSLSFDAVTTVGDKIFFFKDWFFWWRLPGSPATNITSISSMWPTIPSGIQAAYEIGGRNQLFLFKDEKYWLINNLVPEPHYPRSIHSLGFPASVKKIDAAVFDPLRQKVYFFVDKQYWRYDVRQELMDAAYPKLISTHFPGIRPKIDAVLYFKRHYYIFQGAYQLEYDPLLDRVTKTLSSTSWFGC |
| Enzyme Length | 465 |
| Uniprot Accession Number | Q63341 |
| Absorption | |
| Active Site | ACT_SITE 215; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of soluble and insoluble elastin. Specific cleavages are also produced at 14-Ala-|-Leu-15 and 16-Tyr-|-Leu-17 in the B chain of insulin.; EC=3.4.24.65; |
| DNA Binding | |
| EC Number | 3.4.24.65 |
| Enzyme Function | FUNCTION: May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3 (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Disulfide bond (1); Glycosylation (1); Metal binding (23); Motif (1); Propeptide (1); Repeat (4); Sequence conflict (2); Signal peptide (1) |
| Keywords | Calcium;Disulfide bond;Extracellular matrix;Glycoprotein;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Repeat;Secreted;Signal;Zinc;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..21; /evidence=ECO:0000305 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 12626598; 12783419; 15569474; 15802269; 20488952; 21894146; 22223197; 22730688; 23619615; 24907602; 25595645; 25955565; 26534974; 27807143; 29428638; |
| Motif | MOTIF 86..93; /note=Cysteine switch; /evidence=ECO:0000250 |
| Gene Encoded By | |
| Mass | 53,738 |
| Kinetics | |
| Metal Binding | METAL 88; /note=Zinc 2; in inhibited form; /evidence=ECO:0000250; METAL 120; /note=Calcium 1; /evidence=ECO:0000250; METAL 154; /note=Calcium 2; /evidence=ECO:0000250; METAL 164; /note=Zinc 1; /evidence=ECO:0000250; METAL 166; /note=Zinc 1; /evidence=ECO:0000250; METAL 171; /note=Calcium 3; /evidence=ECO:0000250; METAL 172; /note=Calcium 3; via carbonyl oxygen; /evidence=ECO:0000250; METAL 174; /note=Calcium 3; via carbonyl oxygen; /evidence=ECO:0000250; METAL 176; /note=Calcium 3; via carbonyl oxygen; /evidence=ECO:0000250; METAL 179; /note=Zinc 1; /evidence=ECO:0000250; METAL 186; /note=Calcium 2; via carbonyl oxygen; /evidence=ECO:0000250; METAL 190; /note=Calcium 2; /evidence=ECO:0000250; METAL 192; /note=Zinc 1; /evidence=ECO:0000250; METAL 194; /note=Calcium 3; /evidence=ECO:0000250; METAL 195; /note=Calcium 1; /evidence=ECO:0000250; METAL 197; /note=Calcium 1; /evidence=ECO:0000250; METAL 197; /note=Calcium 3; /evidence=ECO:0000250; METAL 214; /note=Zinc 2; catalytic; /evidence=ECO:0000250; METAL 218; /note=Zinc 2; catalytic; /evidence=ECO:0000250; METAL 224; /note=Zinc 2; catalytic; /evidence=ECO:0000250; METAL 285; /note=Calcium 4; via carbonyl oxygen; /evidence=ECO:0000250; METAL 377; /note=Calcium 4; via carbonyl oxygen; /evidence=ECO:0000250; METAL 426; /note=Calcium 4; via carbonyl oxygen; /evidence=ECO:0000250 |
| Rhea ID | |
| Cross Reference Brenda |