| IED ID | IndEnz0002006108 |
| Enzyme Type ID | protease006108 |
| Protein Name |
Outer mitochondrial transmembrane helix translocase EC 7.4.2.- Mitochondrial sorting of proteins Tat-binding homolog 4 |
| Gene Name | MSP1 YTA4 YGR028W |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces Saccharomyces cerevisiae (Baker's yeast) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Enzyme Sequence | MSRKFDLKTITDLSVLVGTGISLYYLVSRLLNDVESGPLSGKSRESKAKQSLQWEKLVKRSPALAEVTLDAYERTILSSIVTPDEINITFQDIGGLDPLISDLHESVIYPLMMPEVYSNSPLLQAPSGVLLYGPPGCGKTMLAKALAKESGANFISIRMSSIMDKWYGESNKIVDAMFSLANKLQPCIIFIDEIDSFLRERSSTDHEVTATLKAEFMTLWDGLLNNGRVMIIGATNRINDIDDAFLRRLPKRFLVSLPGSDQRYKILSVLLKDTKLDEDEFDLQLIADNTKGFSGSDLKELCREAALDAAKEYIKQKRQLIDSGTIDVNDTSSLKIRPLKTKDFTKKLRMDATSTLSSQPLD |
| Enzyme Length | 362 |
| Uniprot Accession Number | P28737 |
| Absorption | |
| Active Site | |
| Activity Regulation | ACTIVITY REGULATION: Interaction with PEX3 inhibits the translocase activity by preventing unfolding of target proteins. {ECO:0000269|PubMed:32541053}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=[protein]-with a C-terminal TM segment(out) + ATP + H2O = [protein]-with a C-terminal TM segment(in) + ADP + H(+) + phosphate; Xref=Rhea:RHEA:66168, Rhea:RHEA-COMP:16963, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:90782, ChEBI:CHEBI:456216; Evidence={ECO:0000269|PubMed:28712723, ECO:0000269|PubMed:28906250, ECO:0000269|PubMed:32541053}; |
| DNA Binding | |
| EC Number | 7.4.2.- |
| Enzyme Function | FUNCTION: Outer mitochondrial translocase required to remove mislocalized tail-anchored transmembrane proteins on mitochondria (PubMed:24843043, PubMed:24821790, PubMed:28906250, PubMed:30858337, PubMed:31445887, PubMed:28712723). Specifically recognizes and binds exposed hydrophobic surfaces of mistargeted tail-anchored transmembrane proteins (PubMed:30858337). Acts as a dislocase that mediates the ATP-dependent extraction of mistargeted tail-anchored transmembrane proteins from the mitochondrion outer membrane (PubMed:24843043, PubMed:24821790, PubMed:28906250, PubMed:30858337, PubMed:32541053, PubMed:28712723). Able to unfold protein substrates by processive threading through its central pore (PubMed:32541053). Once extracted from the mitochondrion outer membrane, substrate proteins are then transferred to the endoplasmic reticulum, where they are ubiquitinated and degraded by the proteasome (PubMed:31445887). Also mediates extraction of excess tail-anchored proteins from the peroxisomal membrane (PubMed:28906250). In normal conditions, MSP1 translocase activity is inhibited by PEX3 at peroxisomes; only catalyzes removal of excess tail-anchored proteins (PubMed:28906250). {ECO:0000269|PubMed:24821790, ECO:0000269|PubMed:24843043, ECO:0000269|PubMed:28712723, ECO:0000269|PubMed:28906250, ECO:0000269|PubMed:30858337, ECO:0000269|PubMed:31445887, ECO:0000269|PubMed:32541053}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 133..140; /note=ATP; /evidence=ECO:0000255 |
| Features | Beta strand (6); Chain (1); Helix (18); Mutagenesis (16); Nucleotide binding (1); Topological domain (2); Transmembrane (1); Turn (3) |
| Keywords | 3D-structure;ATP-binding;Membrane;Mitochondrion;Mitochondrion outer membrane;Nucleotide-binding;Peroxisome;Reference proteome;Translocase;Transmembrane;Transmembrane helix |
| Interact With | Q12421 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Mitochondrion outer membrane {ECO:0000269|PubMed:24843043, ECO:0000269|PubMed:28906250, ECO:0000269|PubMed:31445887, ECO:0000269|PubMed:8226973, ECO:0000305|PubMed:24821790}; Single-pass membrane protein {ECO:0000255}. Peroxisome membrane {ECO:0000269|PubMed:24821790, ECO:0000269|PubMed:24843043, ECO:0000269|PubMed:28906250}; Single-pass membrane protein {ECO:0000255}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 5W0T; |
| Mapped Pubmed ID | 11309190; 16429126; 18467557; 18719252; 18983662; 19930686; 21496646; 21810245; 24391512; 26392202; 27664110; 34857875; 9695811; |
| Motif | |
| Gene Encoded By | |
| Mass | 40,343 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:66168 |
| Cross Reference Brenda |