| IED ID | IndEnz0002006118 |
| Enzyme Type ID | protease006118 |
| Protein Name |
Extracellular metalloproteinase NpIII EC 3.4.24.- Elastinolytic metalloproteinase NpIII Fungalysin NpIII Neutral protease III |
| Gene Name | NpIII AO090138000160 |
| Organism | Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Circumdati Aspergillus oryzae (Yellow koji mold) Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) |
| Enzyme Sequence | MHMLLFIGALALPVFVCTQSCEPASLSPRLAGVDLEKFRLTPNAEYVDSDQQIPISTTNVGLIEQSYVETAIKLVRETFPNATFRLREDHYVGDNGVAHVHFRQTVHNLDVDNGDFNVNVGRDGSVFSYGNSFYTGPVPSITQLTKRDFTDPVAALKFALTHLQLPITAEDVSVESTKHPHKYVLRGTSGAVTNPKARLVYFVKPDGTLCLVWRVETDVDDNWLLTYVDAKTAEEIHGVVDYVSEATFQVYGWGINDPGQVDSRAVLTDPWDLKESPLTWFRDGQKNWTTTRGNNGIAQENINNLPTYLNNFRPDSPTQNFSYEYPAGGSPKDYINASITQLFYTANAYHDLLYTLGFNEKAGNFQWNNSGLGGKEKDYVILNAQDGASRNNADFATPPDGSPARMRMYLFTHTTPPRDGVFESGIVIHEYTHGLSMRLTGGPDNSRCLSAFESASMGEGWGDFMATAIRLKPSDTRATDYGMGMWVYNNEKGIRQYLYSTSMETNPLNYTSLNRMWEAHAGGTVWASMLYEVLWNLIDRHGKNDGPRPTFDERGVPKDGKYLAMKIVIDAMALQPCNPDFVQARNAILDADQALTGGQNKCEIWTGFAKRGLGQGAEYGRGRRVGSYDIPGDVCQKKI |
| Enzyme Length | 639 |
| Uniprot Accession Number | Q2U1N5 |
| Absorption | |
| Active Site | ACT_SITE 430; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.24.- |
| Enzyme Function | FUNCTION: Secreted metalloproteinase that allows assimilation of proteinaceous substrates. {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Glycosylation (5); Metal binding (2); Propeptide (1); Sequence conflict (2); Signal peptide (1) |
| Keywords | Glycoprotein;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Secreted;Signal;Zinc;Zymogen |
| Interact With | |
| Induction | INDUCTION: Expression is controlled by the prtT transcription factor. |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..16; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 71,153 |
| Kinetics | |
| Metal Binding | METAL 429; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 433; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095 |
| Rhea ID | |
| Cross Reference Brenda |