| IED ID | IndEnz0002006119 |
| Enzyme Type ID | protease006119 |
| Protein Name |
Neutral protease 2 EC 3.4.24.39 Deuterolysin Neutral protease II NPII |
| Gene Name | AO090010000493 |
| Organism | Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Circumdati Aspergillus oryzae (Yellow koji mold) Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) |
| Enzyme Sequence | MRVTTLSTALFALASTAVSAPTAGSSSPGLEVKLTQIDNTRVKAVVKNTGSEEVSFVHLNFFKDAGPVKKVSIYRGQDEVQFEGIKRRLRSSGITKEAVTSLGAGETLEDEFDIASTSDLASGGPVSIRSHGFVPIVVDGKITGYIPYKSNDLTVNVDGGKAAKVTKALSQLTRRTEVTDCKGDAESSLTTALSNAAKLANQAAEAAESGDESKFEEYFKTTDQQTRTTVAERLRAVAKEAGSTSGGSTTYHCNDPYGYCEPNVLAYTLPSKNEIANCDIYYSELPPLAQKCHAQDQATTTLHEFTHAPGVYQPGTEDLGYGYDAATQLSAQDALNNADSYALYANAIELKC |
| Enzyme Length | 352 |
| Uniprot Accession Number | P46076 |
| Absorption | |
| Active Site | ACT_SITE 304; /evidence=ECO:0000269|PubMed:11679721 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Preferential cleavage of bonds with hydrophobic residues in P1'. Also 3-Asn-|-Gln-4 and 8-Gly-|-Ser-9 bonds in insulin B chain.; EC=3.4.24.39; |
| DNA Binding | |
| EC Number | 3.4.24.39 |
| Enzyme Function | FUNCTION: Metalloprotease that shows high activities on basic nuclear substrates such as histone and protamine. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Thermostable.; |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (6); Chain (1); Disulfide bond (3); Helix (9); Metal binding (3); Mutagenesis (1); Propeptide (1); Sequence conflict (2); Signal peptide (1); Turn (1) |
| Keywords | 3D-structure;Cleavage on pair of basic residues;Direct protein sequencing;Disulfide bond;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Signal;Zinc;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..19; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 1EB6; |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 37,502 |
| Kinetics | |
| Metal Binding | METAL 303; /note="Zinc; catalytic"; /evidence="ECO:0000269|PubMed:11679721, ECO:0007744|PDB:1EB6"; METAL 307; /note="Zinc; catalytic"; /evidence="ECO:0000269|PubMed:11679721, ECO:0007744|PDB:1EB6"; METAL 318; /note="Zinc; catalytic"; /evidence="ECO:0000269|PubMed:11679721, ECO:0007744|PDB:1EB6" |
| Rhea ID | |
| Cross Reference Brenda | 3.4.24.39; |