| IED ID | IndEnz0002006145 |
| Enzyme Type ID | protease006145 |
| Protein Name |
Peptidoglycan endopeptidase RipA EC 3.4.-.- Resuscitation-promoting factor interaction partner A Rpf-interacting protein A |
| Gene Name | ripA MSMEG_3145 MSMEI_3064 |
| Organism | Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Corynebacteriales Mycobacteriaceae Mycolicibacterium Mycolicibacterium smegmatis (Mycobacterium smegmatis) Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) |
| Enzyme Sequence | MRRTVRALATRVHGRVCAVPLVVGMLLATALYGGGPAAADPAAPDNLATLVAKVASADQKLQELGAAIQTQQETVNKAIVDVQAARDAAAAAQRELEAGQRGVADANAAIEAAQKRFDSFAAATYMNGPSRSYLTATDPADIVNTTATGQALIASSQQVMAKLQRARTEQVNRESAARLAKEKADQAARDAESSQDNAVAALKQAQQTFNAQQGELERLAAERAAAQAELDSVRKVSATGNAAPAAAPAAAPAPAAAPAPVPNSAPAPVPGAQPNPQAAAGNWDRAPSGPASSGQNWAVWDPTLPAIPSAFVSGDPIAIINAVLGIASTSAQVTADMGRSFLQKLGILPTPTGFTNGAIPRVYGREAVEYVIRRGMSQIGVPYSWGGGNAAGPSRGIDSGAGTVGFDCSGLMLYMFAGVGIKLDHYSGSQYNAGRKIPSSQMRRGDMIFYGPNASQHVAMYLGNGQMLEAPYTGSHVKVSPVRTSGMTPYVTRLIEY |
| Enzyme Length | 497 |
| Uniprot Accession Number | A0QX22 |
| Absorption | |
| Active Site | ACT_SITE 408; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU01284; ACT_SITE 457; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01284; ACT_SITE 469; /evidence=ECO:0000255|PROSITE-ProRule:PRU01284 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.-.- |
| Enzyme Function | FUNCTION: Peptidoglycan endopeptidase that cleaves the bond between D-glutamate and meso-diaminopimelate. Binds and degrades high-molecular weight peptidoglycan. Required for normal separation of daughter cells after cell division and for cell wall integrity (By similarity). {ECO:0000250, ECO:0000269|PubMed:18463693}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Compositional bias (1); Domain (1); Region (2); Signal peptide (1) |
| Keywords | Cell wall biogenesis/degradation;Hydrolase;Protease;Reference proteome;Secreted;Signal;Thiol protease |
| Interact With | |
| Induction | INDUCTION: Expression depends on the two-component regulatory system MtrA/MtrB. {ECO:0000269|PubMed:22610443}. |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. Note=Localizes to the septa. {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..39; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 51,317 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |