| IED ID | IndEnz0002006212 |
| Enzyme Type ID | protease006212 |
| Protein Name |
Polyprotein P2A Cleaved into: N-terminal protein; Serine protease EC 3.4.21.- ; VPg; Putative protein p10; Putative protein p8 |
| Gene Name | ORF2A |
| Organism | Southern bean mosaic virus (isolate Bean/United States/Arkansas) (SBMV) |
| Taxonomic Lineage | Viruses Riboviria Orthornavirae Pisuviricota Pisoniviricetes Sobelivirales Solemoviridae Sobemovirus Southern bean mosaic virus Southern bean mosaic virus (isolate Bean/United States/Arkansas) (SBMV) |
| Enzyme Sequence | MYHPGRSPSFLITLANVICAAILFDIHTGGYQPGSLIPIVAWMTPFVTLLWLSASFATYLYKYVRTRLLPEEKVARVYYTAQSAPYFDPALGVMMQFAPSHGGASIEVQVNPSWISLLGGSLKINGDDASNESAVLGSFYSSVKPGDEPASLVAIKSGPQTIGFGCRTKIDGDDCLFTANHVWNNSMRPTALAKRGKQVAIEDWETPLSCDHKMLDFVVVRVPKHVWSKLGVKATQLVCPSDKDAVTCYGGSSSDNLLSGTGVCSKVDFSWKLTHSCPTAAGWSGTPIYSSRGVVGMHVGFEDIGKLNRGVNAFYVSNYLLRSQETLPPELSVIEIPFEDVETRSYEFIEVEIKGRGKAKLGKREFAWIPESGKYWADDDDDSLPPPPKVVDGKMVWSSAQETVAEPLNYQRAAGSRPLPPFLNLQATTSKKEKQPLQEECPLDLLGSRLASLESCVEKILQMKSLELLGSSQNCQTSPGPSEAPKQSFTPCYSKQESLIPLESQGILKELVKTSLSATPPPNPVTVSVEKPGPSTQSTKKSARRRNRRKSTRKPVQESPSPASPQPTKTSLRGI |
| Enzyme Length | 575 |
| Uniprot Accession Number | O73564 |
| Absorption | |
| Active Site | ACT_SITE 181; /note=For protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01216; ACT_SITE 216; /note=For protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01216; ACT_SITE 284; /note=For protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01216 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: [Serine protease]: Responsible for cleavages of polyprotein P2A and replicase polyprotein P2AB.; FUNCTION: [VPg]: Covalently attached to the 5' extremity of the genomic and subgenomic RNAs. It may serve as a primer for the replicase. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (6); Compositional bias (2); Domain (1); Erroneous gene model prediction (2); Region (1); Site (4); Transmembrane (3) |
| Keywords | Covalent protein-RNA linkage;Host membrane;Hydrolase;Membrane;Protease;Reference proteome;Ribosomal frameshifting;Serine protease;Transferase;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: [Polyprotein P2A]: Host membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.; SUBCELLULAR LOCATION: [N-terminal protein]: Host membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | PTM: The polyprotein is proteolytically cleaved into several chains by the viral protease. {ECO:0000305}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 62,722 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |