| IED ID | IndEnz0002006230 |
| Enzyme Type ID | protease006230 |
| Protein Name |
Porin D EC 3.4.21.- Imipenem/basic amino acid-specific outer membrane pore Outer membrane protein D2 |
| Gene Name | oprD PA0958 |
| Organism | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Pseudomonadales Pseudomonadaceae Pseudomonas Pseudomonas aeruginosa group Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) |
| Enzyme Sequence | MKVMKWSAIALAVSAGSTQFAVADAFVSDQAEAKGFIEDSSLDLLLRNYYFNRDGKSGSGDRVDWTQGFLTTYESGFTQGTVGFGVDAFGYLGLKLDGTSDKTGTGNLPVMNDGKPRDDYSRAGGAVKVRISKTMLKWGEMQPTAPVFAAGGSRLFPQTATGFQLQSSEFEGLDLEAGHFTEGKEPTTVKSRGELYATYAGETAKSADFIGGRYAITDNLSASLYGAELEDIYRQYYLNSNYTIPLASDQSLGFDFNIYRTNDEGKAKAGDISNTTWSLAAAYTLDAHTFTLAYQKVHGDQPFDYIGFGRNGSGAGGDSIFLANSVQYSDFNGPGEKSWQARYDLNLASYGVPGLTFMVRYINGKDIDGTKMSDNNVGYKNYGYGEDGKHHETNLEAKYVVQSGPAKDLSFRIRQAWHRANADQGEGDQNEFRLIVDYPLSIL |
| Enzyme Length | 443 |
| Uniprot Accession Number | P32722 |
| Absorption | |
| Active Site | ACT_SITE 179; /evidence=ECO:0000269|PubMed:9636669; ACT_SITE 231; /evidence=ECO:0000269|PubMed:9636669; ACT_SITE 319; /evidence=ECO:0000269|PubMed:9636669 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Porin with a specificity for basic amino acids. Also possesses serine protease activity. {ECO:0000269|PubMed:2118530, ECO:0000269|PubMed:8843159}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Beta strand (21); Chain (1); Helix (3); Mutagenesis (4); Sequence conflict (1); Signal peptide (1); Turn (5) |
| Keywords | 3D-structure;Cell outer membrane;Direct protein sequencing;Hydrolase;Ion transport;Membrane;Porin;Protease;Reference proteome;Serine protease;Signal;Transmembrane;Transmembrane beta strand;Transport |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000269|PubMed:2118530}; Multi-pass membrane protein {ECO:0000269|PubMed:2118530}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..23; /evidence="ECO:0000269|PubMed:1427017, ECO:0000269|PubMed:8012497" |
| Structure 3D | X-ray crystallography (3) |
| Cross Reference PDB | 2ODJ; 3SY7; 4FOZ; |
| Mapped Pubmed ID | 17952093; 22272184; 23467408; |
| Motif | |
| Gene Encoded By | |
| Mass | 48,360 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |