| IED ID | IndEnz0002006233 |
| Enzyme Type ID | protease006233 |
| Protein Name |
Serine protease pet autotransporter EC 3.4.21.- Cleaved into: Serine protease pet Plasmid-encoded toxin pet ; Serine protease pet translocator |
| Gene Name | pet EC042_pAA035 |
| Organism | Escherichia coli O44:H18 (strain 042 / EAEC) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli Escherichia coli O44:H18 Escherichia coli O44:H18 (strain 042 / EAEC) |
| Enzyme Sequence | MNKIYSIKYSAATGGLIAVSELAKKVICKTNRKISAALLSLAVISYTNIIYAANMDISKAWARDYLDLAQNKGVFQPGSTHVKIKLKDGTDFSFPALPVPDFSSATANGAATSIGGAYAVTVAHNAKNKSSANYQTYGSTQYTQINRMTTGNDFSIQRLNKYVVETRGADTSFNYNENNQNIIDRYGVDVGNGKKEIIGFRVGSGNTTFSGIKTSQTYQADLLSASLFHITNLRANTVGGNKVEYENDSYFTNLTTNGDSGSGVYVFDNKEDKWVLLGTTHGIIGNGKTQKTYVTPFDSKTTNELKQLFIQNVNIDNNTATIGGGKITIGNTTQDIEKNKNNQNKDLVFSGGGKISLKENLDLGYGGFIFDENKKYTVSAEGNNNVTFKGAGIDIGKGSTVDWNIKYASNDALHKIGEGSLNVIQAQNTNLKTGNGTVILGAQKTFNNIYVAGGPGTVQLNAENALGEGDYAGIFFTENGGKLDLNGHNQTFKKIAATDSGTTITNSNTTKESVLSVNNQNNYIYHGNVDGNVRLEHHLDTKQDNARLILDGDIQANSISIKNAPLVMQGHATDHAIFRTTKTNNCPEFLCGVDWVTRIKNAENSVNQKNKTTYKSNNQVSDLSQPDWETRKFRFDNLNIEDSSLSIARNADVEGNIQAKNSVINIGDKTAYIDLYSGKNITGAGFTFRQDIKSGDSIGESKFTGGIMATDGSISIGDKAIVTLNTVSSLDRTALTIHKGANVTASSSLFTTSNIKSGGDLTLTGATESTGEITPSMFYAAGGYELTEDGANFTAKNQASVTGDIKSEKAAKLSFGSADKDNSATRYSQFALAMLDGFDTSYQGSIKAAQSSLAMNNALWKVTGNSELKKLNSTGSMVLFNGGKNIFNTLTVDELTTSNSAFVMRTNTQQADQLIVKNKLEGANNLLLVDFIEKKGNDKNGLNIDLVKAPENTSKDVFKTETQTIGFSDVTPEIKQQEKDGKSVWTLTGYKTVANADAAKKATSLMSGGYKAFLAEVNNLNKRMGDLRDINGEAGAWARIMSGTGSAGGGFSDNYTHVQVGADNKHELDGLDLFTGVTMTYTDSHAGSDAFSGETKSVGAGLYASAMFESGAYIDLIGKYVHHDNEYTATFAGLGTRDYSSHSWYAGAEVGYRYHVTDSAWIEPQAELVYGAVSGKQFSWKDQGMNLTMKDKDFNPLIGRTGVDVGKSFSGKDWKVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRMLMNVGLNAEIRDNVRFGLEFEKSAFGKYNVDNAINANFRYSF |
| Enzyme Length | 1295 |
| Uniprot Accession Number | O68900 |
| Absorption | |
| Active Site | ACT_SITE 124; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01028; ACT_SITE 153; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01028; ACT_SITE 260; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01028 |
| Activity Regulation | ACTIVITY REGULATION: Inhibition of cytotoxic activity by phenylmethylsulfonyl fluoride. {ECO:0000269|PubMed:10225873}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Serine protease with enterotoxic and cytotoxic activity. Internalization into the host cell is required for the induction of cytopathic effects. However, the serine activity is not necessary for secretion and internalization into the host cell. {ECO:0000269|PubMed:10225873, ECO:0000269|PubMed:10496914, ECO:0000269|PubMed:11160002, ECO:0000269|PubMed:9632580}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Beta strand (64); Chain (3); Domain (2); Helix (9); Mutagenesis (2); Signal peptide (1); Site (1); Turn (7) |
| Keywords | 3D-structure;Cell outer membrane;Direct protein sequencing;Hydrolase;Membrane;Periplasm;Plasmid;Protease;Secreted;Serine protease;Signal;Transmembrane;Transmembrane beta strand;Virulence;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: [Serine protease pet autotransporter]: Periplasm {ECO:0000250}.; SUBCELLULAR LOCATION: [Serine protease pet]: Secreted. Cell surface.; SUBCELLULAR LOCATION: [Serine protease pet translocator]: Cell outer membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. Note=The cleaved C-terminal fragment (autotransporter domain) is localized in the outer membrane. {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | PTM: Cleaved to release the mature protein from the outer membrane. |
| Signal Peptide | SIGNAL 1..52; /evidence=ECO:0000269|PubMed:9632580 |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 4OM9; |
| Mapped Pubmed ID | 24530907; |
| Motif | |
| Gene Encoded By | Plasmid pAA2 |
| Mass | 139,769 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |