| IED ID | IndEnz0002006238 |
| Enzyme Type ID | protease006238 |
| Protein Name |
Proline iminopeptidase PIP EC 3.4.11.5 Prolyl aminopeptidase PAP Tricorn protease-interacting factor F1 |
| Gene Name | pip TV0934 TVG0960913 |
| Organism | Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1) |
| Taxonomic Lineage | cellular organisms Archaea Candidatus Thermoplasmatota Thermoplasmata Thermoplasmatales Thermoplasmataceae Thermoplasma Thermoplasma volcanium Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1) |
| Enzyme Sequence | MRKLSRCEDGYVKIQGIYIYYKVCKAENEKAKLMTLHGGPGMSHDYLLSLTDLAEKGITVLFYDQFGCGRSEEPEKEKFTIDYGVEEAEAVKKNIFGDDKVFLMGSSYGGALALAYAVKYQAHLKGLIISGGLSSVPLTVKEMQRLIDELPEKYRNAIRKYGEVGDYQNPEYQEAVNYFYHQHLLRSEDWPPEVLKSLEYAEERNVYRTMNGPNEFTITGTIRDWDITDKIGIISVPTLITVGEFDEVTQNVAEVIHSKIDNSQLIVFKACSHLTMWEDRDEYNRILLQFIEKNI |
| Enzyme Length | 295 |
| Uniprot Accession Number | Q97A76 |
| Absorption | |
| Active Site | ACT_SITE 107; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 246; /evidence=ECO:0000250; ACT_SITE 273; /note=Proton donor; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Release of N-terminal proline from a peptide.; EC=3.4.11.5; |
| DNA Binding | |
| EC Number | 3.4.11.5 |
| Enzyme Function | FUNCTION: Cleaves H-Pro-AMC as well as a wide spectrum of amino acid substrates and several peptide substrates without a proline at the N-terminus. In conjunction with the three factors F1, F2 and F3, Tricorn degrades oligopeptides in a sequential manner, yielding free amino acids (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Domain (1) |
| Keywords | Aminopeptidase;Hydrolase;Protease |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 33,830 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |