| IED ID | IndEnz0002006240 |
| Enzyme Type ID | protease006240 |
| Protein Name |
Collagenase EC 3.4.21.49 Hypodermin C HC |
| Gene Name | |
| Organism | Hypoderma lineatum (Early cattle grub) (Common cattle grub) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Calyptratae Oestroidea Oestridae (warble flies) Hypodermatinae Hypoderma Hypoderma lineatum (Early cattle grub) (Common cattle grub) |
| Enzyme Sequence | MKFLLVFALALATTSAFQHPASIFELREGRIINGYEAYTGLFPYQAGLDITLQDQRRVWCGGSLIDNKWILTAAHCVHDAVSVVVYLGSAVQYEGEAVVNSERIISHSMFNPDTYLNDVALIKIPHVEYTDNIQPIRLPSGEELNNKFENIWATVSGWGQSNTDTVILQYTYNLVIDNDRCAQEYPPGIIVESTICGDTCDGKSPCFGDSGGPFVLSDKNLLIGVVSFVSGAGCESGKPVGFSRVTSYMDWIQQNTGIIF |
| Enzyme Length | 260 |
| Uniprot Accession Number | P08897 |
| Absorption | |
| Active Site | ACT_SITE 75; /note=Charge relay system; ACT_SITE 118; /note=Charge relay system; ACT_SITE 210; /note=Charge relay system |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by diisopropylfluorophosphate. {ECO:0000269|PubMed:230030}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of proteins including native collagen at Xaa-|-Ala bond leaving an N-terminal (75%) and a C-terminal (25%) fragment.; EC=3.4.21.49; Evidence={ECO:0000269|PubMed:230030, ECO:0000269|PubMed:2995028}; |
| DNA Binding | |
| EC Number | 3.4.21.49 |
| Enzyme Function | FUNCTION: This enzyme is a serine protease capable of degrading the native triple helix of collagen. Also cleaves the B chain of insulin at the 15-Leu-|-Try-16 and 22-Arg-|-Gly-23 bonds. Hydrolyzes casein, but not Px-Pro-Leu-Gly-Pro-DArg, BzArgNHPh, AcTyrNHPh, 2-naphthyl phosphate, 2-naphthyl butyrate, 2-naphthyl caprylate, 2-naphthyl myristate, L-leucine 2-2-naphthylamide, L-valine 2-naphthylamide, L-cysteine 2-naphthylamide or L-glutarylphenylalanine 2-naphthylamide. {ECO:0000269|PubMed:230030, ECO:0000269|PubMed:2995028}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Thermostable. No loss of activity occurs after incubation for 2 hours at 60 degrees Celsius. Inactivated after incubation for 2 hours at 75 degrees Celsius, however 45% of activity remains after incubation for 20 minutes at 75 degrees Celsius. {ECO:0000269|PubMed:2995028}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 8.0-8.5. Reversibly inactivated below pH 4.5. {ECO:0000269|PubMed:2995028}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Beta strand (13); Chain (1); Disulfide bond (3); Domain (1); Helix (5); Propeptide (1); Sequence conflict (6); Signal peptide (1); Turn (2) |
| Keywords | 3D-structure;Collagen degradation;Direct protein sequencing;Disulfide bond;Hydrolase;Protease;Secreted;Serine protease;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..16; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (2) |
| Cross Reference PDB | 1HYL; 2HLC; |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 28,579 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.4.21.49; |