| IED ID | IndEnz0002006256 |
| Enzyme Type ID | protease006256 |
| Protein Name |
Hatching enzyme 1.2 EC 3.4.24.67 Choriolysin H homolog 1 High choriolytic enzyme 1 homolog zHCE-1 |
| Gene Name | he1.2 he1 he1a he1b |
| Organism | Danio rerio (Zebrafish) (Brachydanio rerio) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Actinopterygii Actinopteri Neopterygii Teleostei Osteoglossocephalai Clupeocephala Otomorpha Ostariophysi Otophysi Cypriniphysae Cypriniformes (carps and others) Cyprinoidei Danionidae Danioninae Danio Danio rerio (Zebrafish) (Brachydanio rerio) |
| Enzyme Sequence | MDIRASLSILLLLFGLSQASPLREFEAIFVSEPETVDITTQILETNKGSSEVLFEGDVVLPKNRNALICEDKSCFWKKNANNIVEVPYVVSGEFSINDKSVIANAISIFHAQTCIRFVPRSIQADYLSIENKDGCYSAIGRTGGKQVVSLNRKGCVYSGIAQHELNHALGFYHEQSRSDRDQYVRINWNNISPGMAYNFLKQKTNNQNTPYDYGSLMHYGKTAFAIQPGLETITPIPDENVQIGQRQGLSKIDILRINKLYGC |
| Enzyme Length | 263 |
| Uniprot Accession Number | Q1LW01 |
| Absorption | |
| Active Site | ACT_SITE 164; /evidence=ECO:0000255|PROSITE-ProRule:PRU01211 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of the inner layer of fish egg envelope. Also hydrolysis of casein and small molecule substrates such as succinyl-Leu-Leu-Val-Tyr-|-7-(4-methyl)coumarylamide.; EC=3.4.24.67; Evidence={ECO:0000269|PubMed:19021768, ECO:0000269|PubMed:20727360}; |
| DNA Binding | |
| EC Number | 3.4.24.67 |
| Enzyme Function | FUNCTION: Metalloendopeptidase which participates in the breakdown of the egg envelope at the time of hatching. Cleaves the N-terminal regions of the zona pellucia glycoproteins ZP2 and ZP3, where it specifically recognizes the peptide sequences TVQQS-|-DYLIK (major site) and KLMLK-|-APEPF (minor site). {ECO:0000269|PubMed:19021768, ECO:0000269|PubMed:20727360}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (8); Chain (1); Disulfide bond (3); Domain (1); Helix (7); Metal binding (3); Propeptide (1); Sequence conflict (2); Signal peptide (1); Turn (2) |
| Keywords | 3D-structure;Direct protein sequencing;Disulfide bond;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Secreted;Signal;Zinc;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:19021768}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..19; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 3LQB; |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 29,448 |
| Kinetics | |
| Metal Binding | METAL 163; /note="Zinc; via tele nitrogen; catalytic"; /evidence="ECO:0000269|PubMed:20727360, ECO:0007744|PDB:3LQB"; METAL 167; /note="Zinc; via tele nitrogen; catalytic"; /evidence="ECO:0000269|PubMed:20727360, ECO:0007744|PDB:3LQB"; METAL 173; /note="Zinc; via tele nitrogen; catalytic"; /evidence="ECO:0000269|PubMed:20727360, ECO:0007744|PDB:3LQB" |
| Rhea ID | |
| Cross Reference Brenda |