| IED ID | IndEnz0002006286 |
| Enzyme Type ID | protease006286 |
| Protein Name |
Outer membrane protease IcsP EC 3.4.23.- |
| Gene Name | icsP sopA CP0271 |
| Organism | Shigella flexneri |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Shigella Shigella flexneri |
| Enzyme Sequence | MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGKTKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMVDKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYSFNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAELKYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISNKKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF |
| Enzyme Length | 315 |
| Uniprot Accession Number | O33641 |
| Absorption | |
| Active Site | ACT_SITE 103; /evidence=ECO:0000250; ACT_SITE 105; /evidence=ECO:0000250; ACT_SITE 230; /evidence=ECO:0000250; ACT_SITE 232; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.23.- |
| Enzyme Function | FUNCTION: Protease responsible for the cleavage of IcsA between 'Arg-758' and 'Arg-759', removing the entire alpha domain from IscA localized on the bacterial surface. This proteolytic activity contributes to the maintenance of a tight polar cap of IcsA, which is important to Shigella actin-based motility. {ECO:0000269|PubMed:10231492, ECO:0000269|PubMed:9076742, ECO:0000269|PubMed:9302008}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (4); Chain (1); Erroneous initiation (2); Natural variant (4); Signal peptide (1) |
| Keywords | Aspartyl protease;Cell outer membrane;Hydrolase;Membrane;Plasmid;Protease;Reference proteome;Signal;Transmembrane;Transmembrane beta strand;Virulence |
| Interact With | |
| Induction | INDUCTION: Transcriptionally regulated by VirB (InvE). |
| Subcellular Location | SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000269|PubMed:10231492}; Multi-pass membrane protein {ECO:0000269|PubMed:10231492}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..20; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | Plasmid pWR100; Plasmid pCP301; Plasmid pINV_F6_M1382 |
| Mass | 35,597 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |