| IED ID | IndEnz0002006325 |
| Enzyme Type ID | protease006325 |
| Protein Name |
Probable ubiquitin carboxyl-terminal hydrolase MINDY-4 EC 3.4.19.12 Probable deubiquitinating enzyme MINDY-4 |
| Gene Name | MINDY4 FAM188B |
| Organism | Bos taurus (Bovine) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Laurasiatheria Artiodactyla Ruminantia Pecora Bovidae Bovinae Bos (oxen cattle) Bos taurus (Bovine) |
| Enzyme Sequence | MDTLFVEEVAASLIREFLSRKGLKKTYVTMDQERPRSDLSINSRNDLRKVLHLEFLYKENKAKENPLKTNLELITRYFLDHFGNIGNNVTQETRIPELSVPKKSNKLPLRSSETTLVNIYHLADEDETWRTSLSEISKARHDSLDGDVLGHFVSSKRSSHKSRPIKTVAGESPTVASAWEKTDKLPMSEPSLDTKRMGEKVRPKSGLIVRGMMAGPIASSPQDSLRKRSLRRSPALSSATQPHKEGSPQEPELSTHTSTCPTPLEGPASSTASTSRSPQGPLSELTWEKQRTSPGSPPHLPGKGLLPRGSGRWRDLSEDSPAVDSGSEAIRTPPKFSLSSGNVPKTQERPERAFERQGSQPASLRKNQLSVSNKLEGDLDVLQLEDVEDELVREEIILSPVSSVLKLQVVSKPIDLSVAKDIKTILFGSSFCCFSDEWKLQSFSFNDSVSLKYGIVQNKGGPCGVLAAVQGCVLQKLLFEGDSSADCARLQPSNARRTHCLALAIADIVWRAGGCERAVVTLASGTQHFSPTGKYKADGVLETLILHSLTCYEELVTFLQQSIHQFEAGPYGCVLLTLSAILSRSTELVRQDFDVPTSHLIGAHGYCTQELVNLLLTGKAVSNVFNDVVELDSGNGDVTLLKGISTRSDIGFLSLFEHYNVCQVGCFLKTPRFPIWVVCSESHFSVLFSQQLELLRDWRAERLFDLYYYDGLANQQEQIRLTVDTTQTVPEDRDNGLVPPLELCNRTKWKGASVNWNGSEPIL |
| Enzyme Length | 763 |
| Uniprot Accession Number | A1A4L4 |
| Absorption | |
| Active Site | ACT_SITE 463; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q8N5J2; ACT_SITE 683; /note=Proton acceptor; /evidence=ECO:0000250|UniProtKB:Q8N5J2 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; Evidence={ECO:0000250|UniProtKB:Q8NBR6}; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Probable hydrolase that can remove 'Lys-48'-linked conjugated ubiquitin from proteins. {ECO:0000250|UniProtKB:Q8NBR6}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Compositional bias (3); Modified residue (4); Region (1) |
| Keywords | Hydrolase;Phosphoprotein;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | MOD_RES 143; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:Q3UQI9; MOD_RES 220; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:Q4G0A6; MOD_RES 224; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:Q4G0A6; MOD_RES 296; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:Q4G0A6 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 84,447 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |