| IED ID | IndEnz0002006351 |
| Enzyme Type ID | protease006351 |
| Protein Name |
Pyrolysin EC 3.4.21.- |
| Gene Name | pls PF0287 |
| Organism | Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) |
| Taxonomic Lineage | cellular organisms Archaea Euryarchaeota Thermococci Thermococcales Thermococcaceae Pyrococcus Pyrococcus furiosus Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) |
| Enzyme Sequence | MNKKGLTVLFIAIMLLSVVPVHFVSAGTPPVSSENSTTSILPNQQVVTKEVSQAALNAIMKGQPNMVLIIKTKEGKLEEAKTELEKLGAEILDENRVLNMLLVKIKPEKVKELNYISSLEKAWLNREVKLSPPIVEKDVKTKEPSLEPKMYNSTWVINALQFIQEFGYDGSGVVVAVLDTGVDPNHPFLSITPDGRRKIIEWKDFTDEGFVDTSFSFSKVVNGTLIINTTFQVASGLTLNESTGLMEYVVKTVYVSNVTIGNITSANGIYHFGLLPERYFDLNFDGDQEDFYPVLLVNSTGNGYDIAYVDTDLDYDFTDEVPLGQYNVTYDVAVFSYYYGPLNYVLAEIDPNGEYAVFGWDGHGHGTHVAGTVAGYDSNNDAWDWLSMYSGEWEVFSRLYGWDYTNVTTDTVQGVAPGAQIMAIRVLRSDGRGSMWDIIEGMTYAATHGADVISMSLGGNAPYLDGTDPESVAVDELTEKYGVVFVIAAGNEGPGINIVGSPGVATKAITVGAAAVPINVGVYVSQALGYPDYYGFYYFPAYTNVRIAFFSSRGPRIDGEIKPNVVAPGYGIYSSLPMWIGGADFMSGTSMATPHVSGVVALLISGAKAEGIYYNPDIIKKVLESGATWLEGDPYTGQKYTELDQGHGLVNVTKSWEILKAINGTTLPIVDHWADKSYSDFAEYLGVDVIRGLYARNSIPDIVEWHIKYVGDTEYRTFEIYATEPWIKPFVSGSVILENNTEFVLRVKYDVEGLEPGLYVGRIIIDDPTTPVIEDEILNTIVIPEKFTPENNYTLTWYDINGPEMVTHHFFTVPEGVDVLYAMTTYWDYGLYRPDGMFVFPYQLDYLPAAVSNPMPGNWELVWTGFNFAPLYESGFLVRIYGVEITPSVWYINRTYLDTNTEFSIEFNITNIYAPINATLIPIGLGTYNASVESVGDGEFFIKGIEVPEGTAELKIRIGNPSVPNSDLDLYLYDSKGNLVALDGNPTAEEEVVVEYPKPGVYSIVVHGYSVRDENGNPTTTTFDLVVQMTLDNGNIKLDKDSIILGSNESVVVTANITIDRDHPTGVYSGIIEIRDNEVYQDTNTSIAKIPITLVIDKADFAVGLTPAEGVLGEARNYTLIVKHALTLEPVPNATVIIGNYTYLTDENGTVTFTYAPTKLGSDEITVIVKKENFNTLEKTFQITVSEPEITEEDINEPKLAMSSPEANATIVSVEMESEGGVKKTVTVEITINGTANETATIVVPVPKKAENIEVSGDHVISYSIEEGEYAKYVIITVKFASPVTVTVTYTIYAGPRVSILTLNFLGYSWYRLYSQKFDELYQKALELGVDNETLALALSYHEKAKEYYEKALELSEGNIIQYLGDIRLLPPLRQAYINEMKAVKILEKAIEELEGEE |
| Enzyme Length | 1398 |
| Uniprot Accession Number | P72186 |
| Absorption | |
| Active Site | ACT_SITE 179; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240; ACT_SITE 365; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240; ACT_SITE 590; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Has endopeptidase activity toward caseins, casein fragments including alpha-S1-casein and synthetic peptides. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Thermostable. Highly active at 95 degrees Celsius.; |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Domain (1); Glycosylation (28); Propeptide (1); Sequence conflict (2); Signal peptide (1) |
| Keywords | Direct protein sequencing;Glycoprotein;Hydrolase;Protease;Reference proteome;Serine protease;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell envelope. Note=Cell envelope associated. |
| Modified Residue | |
| Post Translational Modification | PTM: LWM pyrolysin seems to be produced by autoproteolytic activation of HMW pyrolysin.; PTM: Glycosylated. |
| Signal Peptide | SIGNAL 1..26; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 154,475 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.4.21.B55; |