| IED ID | IndEnz0002006389 |
| Enzyme Type ID | protease006389 |
| Protein Name |
Serine protease Hip1 EC 3.4.21.- |
| Gene Name | hip1 BQ2027_MB2248C |
| Organism | Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Corynebacteriales Mycobacteriaceae Mycobacterium Mycobacterium tuberculosis complex Mycobacterium tuberculosis Mycobacterium bovis Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) |
| Enzyme Sequence | MGMRLSRRDKIARMLLIWAALAAVALVLVGCIRVVGGRARMAEPKLGQPVEWTPCRSSNPQVKIPGGALCGKLAVPVDYDRPDGDVAALALIRFPATGDKIGSLVINPGGPGESGIEAALGVFQTLPKRVHERFDLVGFDPRGVASSRPAIWCNSDADNDRLRAEPQVDYSREGVAHIENETKQFVGRCVDKMGKNFLAHVGTVNVAKDLDAIRAALGDDKLTYLGYSYGTRIGSAYAEEFPQRVRAMILDGAVDPNADPIEAELRQAKGFQDAFNNYAADCAKNAGCPLGADPAKAVEVYHSLVDPLVDPDNPRISRPARTKDPRGLSYSDAIVGTIMALYSPNLWQHLTDGLSELVDNRGDTLLALADMYMRRDSHGRYNNSGDARVAINCVDQPPVTDRDKVIDEDRRAREIAPFMSYGKFTGDAPLGTCAFWPVPPTSQPHAVSAPGLVPTVVVSTTHDPATPYKAGVDLANQLRGSLLTFDGTQHTVVFQGDSCIDEYVTAYLIGGTTPPSGAKC |
| Enzyme Length | 520 |
| Uniprot Accession Number | P65824 |
| Absorption | |
| Active Site | ACT_SITE 228; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:P9WHR3; ACT_SITE 463; /evidence=ECO:0000250|UniProtKB:P9WHR3; ACT_SITE 490; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:P9WHR3 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Serine protease that promotes pathogenesis by promoting the processing and the extracellular release of the M.bovis heat-shock protein GroEL2. {ECO:0000250|UniProtKB:P9WHR3}.; FUNCTION: Key immunomodulatory virulence factor, which promotes survival in host macrophages and modulates host immune responses. {ECO:0000250|UniProtKB:P9WHR3}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Domain (1); Lipidation (2); Signal peptide (1) |
| Keywords | Cell membrane;Hydrolase;Lipoprotein;Membrane;Palmitate;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell envelope {ECO:0000250|UniProtKB:P9WHR3}. Cell membrane {ECO:0000255|PROSITE-ProRule:PRU00303}; Lipid-anchor {ECO:0000255|PROSITE-ProRule:PRU00303}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..30; /evidence=ECO:0000255|PROSITE-ProRule:PRU00303 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 55,924 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |