| IED ID | IndEnz0002006414 |
| Enzyme Type ID | protease006414 |
| Protein Name |
Halolysin EC 3.4.21.- Halophilic serine protease |
| Gene Name | hly HFX_2419 C439_13889 |
| Organism | Haloferax mediterranei (strain ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4) (Halobacterium mediterranei) |
| Taxonomic Lineage | cellular organisms Archaea Euryarchaeota Stenosarchaea group Halobacteria Haloferacales Haloferacaceae Haloferax Haloferax mediterranei (Halobacterium mediterranei) Haloferax mediterranei (strain ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4) (Halobacterium mediterranei) |
| Enzyme Sequence | MAGTPNFDRRSFLRLAAAAGLTGMAGVTSATPGRSPGPKKDEILVGVTSTADSPRKAVADAVPGNAEIVHENETLSYAAVKFPSKAPKQARENFISAITKRDEVKYAEKNATHEALYTANDPKYGSQYAPQQVNADSAWDTTLGSSSVKIAVVDQGVKYDHPDLSSQFGSNKGRDFVDNDGDPYPDLLSDEYHGTHVAGIAAGTTDNNEGIGGISNSTLLSGRALSESGSGSTSDIADAIEWAADQGADVINLSLGGGGYSSTMKNAVSYATQQGSLVVAAAGNDGRQSVSYPAAYSECVAVSALDPDETLASYSNYGSEIDLAAPGTNVLSCWTTSTEYNEISGTSMATPVVSGVAGLALAVHNLSPADLRNHLKNTAVDIGLSSTKQGSGRVDAANAVTTDPGDGGGGGGGGSKETTYDGTLSSSSDSNCVSHSWNYSSPSQVVIDLSGPSSADFDLYATEGSGTCPTTRSYDYRSWSYDSTEQIVIDNPDTSADLGILVDSYSGSGSYTVTITEKE |
| Enzyme Length | 519 |
| Uniprot Accession Number | I3R794 |
| Absorption | |
| Active Site | ACT_SITE 154; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240; ACT_SITE 193; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240; ACT_SITE 347; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Probable secreted halophilic serine protease showing proteolytic activity toward the protease general substrate azocasein. {ECO:0000269|PubMed:8645734}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Domain (1); Mutagenesis (2); Propeptide (1); Region (1); Sequence conflict (2); Signal peptide (1) |
| Keywords | Direct protein sequencing;Hydrolase;Protease;Secreted;Serine protease;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | PTM: Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. |
| Signal Peptide | SIGNAL 1..27; /note=Tat-type signal; /evidence=ECO:0000255|PROSITE-ProRule:PRU00648 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 53,516 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |