| IED ID | IndEnz0002006445 |
| Enzyme Type ID | protease006445 |
| Protein Name |
Metacaspase-3 EC 3.4.22.- TbMCA3 Cleaved into: Large subunit p20; Small subunit p10 |
| Gene Name | MCA3 |
| Organism | Trypanosoma brucei brucei |
| Taxonomic Lineage | cellular organisms Eukaryota Discoba Euglenozoa Kinetoplastea (kinetoplasts) Metakinetoplastina Trypanosomatida Trypanosomatidae Trypanosoma Trypanozoon Trypanosoma brucei Trypanosoma brucei brucei |
| Enzyme Sequence | MAVDPRCLLSLCSTISKASSAKGTNDFVKIGMELWQTAQPYLVQALGLQPPPPKVDVDAAVANAGDAHGEQPWVATPLPGQTVRALFIGINYYGTSAALSGCCNDVKQMLATLQKKGLPINEAVILVDEDNFPGRTDQPTRDNIVRYMAWLVKDAKPGDVLFFHYSGHGTQCKSRGDSDEKYDQCIAPVDFQKSGCIVDDDIHKLLFSRLPEKVRLTAVFDCCHSGSIMDLPFTYVCSGGEQASGTPHMKRIREGNDVLGDVMMISGCADEQTSADVKNTATFGTGSTGAGGAATQCITCMLMNNQSLSYGKLLIETRDMLKRKRFKQVPQLSASKAIDLDQTFSLTEMFSVDRSVQ |
| Enzyme Length | 357 |
| Uniprot Accession Number | Q8T8E6 |
| Absorption | |
| Active Site | ACT_SITE 168; /evidence=ECO:0000250|UniProtKB:Q08601; ACT_SITE 223; /evidence=ECO:0000250|UniProtKB:Q8T8E7 |
| Activity Regulation | ACTIVITY REGULATION: Activated by Ca(2+). {ECO:0000305|PubMed:21949125}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.22.- |
| Enzyme Function | FUNCTION: Cysteine protease that cleaves specifically after arginine or lysine residues (PubMed:21949125). In the bloodstream form, may cleave inactive metacaspase-4 MCA4 prior to MCA4 secretion (PubMed:21949125). {ECO:0000269|PubMed:21949125}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (3); Metal binding (4); Propeptide (1); Site (1) |
| Keywords | Autocatalytic cleavage;Calcium;Endosome;Hydrolase;Metal-binding;Protease;Thiol protease;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Recycling endosome {ECO:0000305|PubMed:16507595}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 38,724 |
| Kinetics | |
| Metal Binding | METAL 183; /note=Calcium; /evidence=ECO:0000250|UniProtKB:Q585F3; METAL 199; /note=Calcium; /evidence=ECO:0000250|UniProtKB:Q585F3; METAL 200; /note=Calcium; /evidence=ECO:0000250|UniProtKB:Q585F3; METAL 230; /note=Calcium; /evidence=ECO:0000250|UniProtKB:Q585F3 |
| Rhea ID | |
| Cross Reference Brenda |