| IED ID | IndEnz0002006703 |
| Enzyme Type ID | protease006703 |
| Protein Name |
Protein P1 69.7 kDa protein Genome-linked protein precursor Protein ORF1 Cleaved into: Serine protease EC 3.4.21.- ; VPg/P1-C25 |
| Gene Name | ORF1 |
| Organism | Potato leafroll virus (strain Potato/Scotland/strain 1/1984) (PLrV) |
| Taxonomic Lineage | Viruses Riboviria Orthornavirae Pisuviricota Pisoniviricetes Sobelivirales Solemoviridae Polerovirus Potato leafroll virus (PLrV) Potato leafroll virus (strain Potato/Scotland/strain 1/1984) (PLrV) |
| Enzyme Sequence | MNRFTAYAALFFMFSLCSTAKEAGFLHPAFNFRGTSTMSASSGDYSAAPTPLYKSWALPSSLNLTTQPPPPLTDRSYYELVQALTSKMRLDCQTVGDMTWRHLSEMLFASWNSVKEVSLKAASVTLWAIINIWFGLYWTLARLITLFLWTFSIEALCLILLGCITSLIYKGALSLSEHLPVFLFMSPLKIIWRAAFSKRNYKNERAVEGYKGFSVPQKPPKSAVIELQHENGSHLGYANCIRLYSGENALVTAEHCLEGAFATSLKTGNRIPMSTFFPIFKSARNDISILVGPPNWEGLLSVKGAHFITADKIGKGPASFYTLEKGEWMCHSATIDGAHHQFVSVLCNTGPGYSGTGFWSSKNLLGVLKGFPLEEECNYNVMSVIPSIPGITSPNYVFESTAVKGRVFSDEAVKELEREASEAVKKLARFKSLTDKNWADDYDSDEDYGLEREAATNAPAEKTAQTNSAEKTAPSTSAEKTALTNKPLNGQAAPSAKTNGNSDIPDAATSAPPMDKMVEQIITAMVGRINLSEIEEKIVSRVSQKALQKPKQKKRGRRGGKNKQNSLPPTSTQSTSGAPKKEAAPQASGSAGTSRATTTPAPEAKPSGGKNSAKFTPSWRIKQQDSAGQKPDLKLNSKA |
| Enzyme Length | 639 |
| Uniprot Accession Number | P17519 |
| Absorption | |
| Active Site | ACT_SITE 255; /note="For protease activity"; /evidence="ECO:0000255|PROSITE-ProRule:PRU01216, ECO:0000305|PubMed:11369899"; ACT_SITE 286; /note="For protease activity"; /evidence="ECO:0000255|PROSITE-ProRule:PRU01216, ECO:0000305|PubMed:11369899"; ACT_SITE 354; /note="For protease activity"; /evidence="ECO:0000255|PROSITE-ProRule:PRU01216, ECO:0000305|PubMed:11369899" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Precursor from which the VPg molecule is probably released at the onset of the RNA synthesis. Essential for virus replication. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (3); Compositional bias (3); Domain (1); Mutagenesis (3); Region (2); Signal peptide (1); Site (2); Transmembrane (3) |
| Keywords | Direct protein sequencing;Hydrolase;Membrane;Protease;RNA-binding;Reference proteome;Ribosomal frameshifting;Serine protease;Signal;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: [Protein P1]: Membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | PTM: Specific enzymatic cleavages in vivo yield mature proteins. The protease probably cleaves itself and releases the VPg protein. The VPg protein is probably further cleaved in its C-terminus. {ECO:0000269|PubMed:9268161}. |
| Signal Peptide | SIGNAL 1..20; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 69,629 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |