| IED ID | IndEnz0002006721 |
| Enzyme Type ID | protease006721 |
| Protein Name |
Vacuolar membrane protease EC 3.4.-.- FXNA-related family protease 1 |
| Gene Name | GLRG_01223 |
| Organism | Colletotrichum graminicola (strain M1.001 / M2 / FGSC 10212) (Maize anthracnose fungus) (Glomerella graminicola) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Hypocreomycetidae Glomerellales Glomerellaceae Colletotrichum Colletotrichum graminicola species complex Colletotrichum graminicola (Maize anthracnose fungus) (Glomerella graminicola) Colletotrichum graminicola (strain M1.001 / M2 / FGSC 10212) (Maize anthracnose fungus) (Glomerella graminicola) |
| Enzyme Sequence | MAVKNPFGFTTGPVTFWLIVVYAAFLIPLVWIHESVPAVPSSSKNPYPQLNVTEAWHDLTHITRKYHPYNSRANDEVGAYFMKRIEEILVRNKVEYTKEKDAGGVIWPQEAYDAPAPAKRDVQRRDSKGPAVTIFDDTISNTTYLGTSGSFNQAGAYGTYFEGTNKLVYIRGTLDEDGEWWNGKRDPRKIGQGGVLVNAHYDSVSSGYGATDDGMGCVSILQILNHYTSPGHQPMRGIVLLLNNGEEDGLYGAKVYHYSPLYYFTTSFVNLEGAGAGGRAILFRTTDLEVTKGYEGAPHPFGSVVAADGFKLGAIRSETDYKVWTESYGQRGLDIAFYRPRARYHTNQDDTRHASQESLWHMLSNSLAAVDNLQHTTGYFSGSRNDGDKKKVASGSGTDGVWFDMFGTGFAILELRGLFAWTLTLLIVSPLVLALVTYILSRKDKYYFFSRKVTADEDDEPVSVGGWKGFFRFPFALVLSASITVLSAFLIRRVNPHIIYSSPYAVWAMTLSLFFLVFWTIAKGASVVRPSALQRGYAHIWLFVISWVILVAVTAAADRFKIASGYPFAFFHSAVFVSALISLCDLFALPSKQEFARNAHNDQQTRDNISEVPNSDALISSRHSHVEDDVAEEPTETTPLRSGENGNGNNGTIRTTFATTYRRSLSAIMRTPDEEENKETEQQPYDHEQQWSANLPSWTWFFQLLLLAPITITVFLQIALFIVSAIHSAAADGNDPILVYAAIAAFSIIILLPATPFIHRASFYLPLFLLLVFFVTLIYNLVAFPFSAENRLKVRFQQTLDLDANTSVISITGLEKYTRKMIAELPSGAGQPVNCTPGYGLTSDCRYNGAAVMPNLPQFTHALPANVSKGRYANLVTVNISRSDSSSKLEFGIDAAESKVCNLAFDTPVSFNVRGSAGIDPIFSRPTEQGVRMLTLWRRDASIPWVVDVEPIKTKAVPAGVLAKSSDSDAGAVHEDLRVRKSAELSGTIACQWSDANVLGTIPAFDEALQFSPDWIAVTKASVGLVEGRKKFRA |
| Enzyme Length | 1034 |
| Uniprot Accession Number | E3Q4R4 |
| Absorption | |
| Active Site | ACT_SITE 246; /note=Proton acceptor; /evidence=ECO:0000250|UniProtKB:P80561 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.-.- |
| Enzyme Function | FUNCTION: May be involved in vacuolar sorting and osmoregulation. {ECO:0000250|UniProtKB:P38244}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Compositional bias (1); Glycosylation (5); Metal binding (6); Region (1); Site (1); Topological domain (10); Transmembrane (9) |
| Keywords | Glycoprotein;Hydrolase;Membrane;Metal-binding;Metalloprotease;Protease;Reference proteome;Transmembrane;Transmembrane helix;Vacuole;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000250|UniProtKB:P38244}; Multi-pass membrane protein {ECO:0000255}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 114,272 |
| Kinetics | |
| Metal Binding | METAL 200; /note=Zinc 1; catalytic; /evidence=ECO:0000250|UniProtKB:P80561; METAL 212; /note=Zinc 1; catalytic; /evidence=ECO:0000250|UniProtKB:P80561; METAL 212; /note=Zinc 2; catalytic; /evidence=ECO:0000250|UniProtKB:P80561; METAL 247; /note=Zinc 2; catalytic; /evidence=ECO:0000250|UniProtKB:P80561; METAL 272; /note=Zinc 1; catalytic; /evidence=ECO:0000250|UniProtKB:P80561; METAL 345; /note=Zinc 2; catalytic; /evidence=ECO:0000250|UniProtKB:P80561 |
| Rhea ID | |
| Cross Reference Brenda |