| IED ID | IndEnz0002006732 |
| Enzyme Type ID | protease006732 |
| Protein Name |
Peptidyl-Lys metalloendopeptidase MEP EC 3.4.24.20 GfMEP |
| Gene Name | MEP |
| Organism | Grifola frondosa (Maitake) (Polyporus frondosus) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Basidiomycota Agaricomycotina Agaricomycetes Agaricomycetes incertae sedis Polyporales Grifolaceae Grifola Grifola frondosa (Maitake) (Polyporus frondosus) |
| Enzyme Sequence | MFSSVMVALVSLAVAVSANPGLSLKVSGPEAVDGVNNLKVVTTITNTGDETLKLLNDPRGALHTMPTDTFAITNESGETPSFIGVKVKYVPSMAAKSTGENVFAVIAPGQSVNVEHDLSAAYNFTSSGAGTYALEALNVFNYIDPETNEPVEIWADAEAHTTAVSGKLAVVRATPTLTRPVTYNGCSSSEQSALAAAASAAQSYVAESLSYLQTHTAATPRYTTWFGSYISSRHSTVLQHYTDMNSNDFSSYSFDCTCTAAGTFAYVYPNRFGTVYLCGAFWKAPTTGTDSQAGTLVHESSHFTRNGGTKDYAYGQAAAKSLATMDPDKAVMNADNHEYFSENNPAQS |
| Enzyme Length | 348 |
| Uniprot Accession Number | P81054 |
| Absorption | |
| Active Site | ACT_SITE 299; /evidence=ECO:0000269|PubMed:11223512 |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by chelating agents such as EDTA and 1,10-phenanthroline. {ECO:0000269|PubMed:8749321}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Preferential cleavage in proteins: -Xaa-|-Lys- (in which Xaa may be Pro).; EC=3.4.24.20; |
| DNA Binding | |
| EC Number | 3.4.24.20 |
| Enzyme Function | |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Thermostable for 3 hours up to 80 degrees Celsius. {ECO:0000269|PubMed:8749321}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 9.5. Active from pH 6 to pH 10.5. Stable from pH 5 to pH 10. {ECO:0000269|PubMed:8749321}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (4); Chain (1); Disulfide bond (2); Glycosylation (1); Helix (10); Metal binding (3); Propeptide (1); Signal peptide (1); Site (1) |
| Keywords | 3D-structure;Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Metal-binding;Metalloprotease;Protease;Secreted;Signal;Zinc;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000305}. Note=Binds strongly to beta-1,3-glucan and chitin, major polysaccharides constituting the fungal cell wall. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..18; /evidence=ECO:0000269|PubMed:12501439 |
| Structure 3D | X-ray crystallography (4) |
| Cross Reference PDB | 1G12; 1GE5; 1GE6; 1GE7; |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 36,879 |
| Kinetics | |
| Metal Binding | METAL 298; /note=Zinc; catalytic; /evidence=ECO:0000269|PubMed:11223512; METAL 302; /note=Zinc; catalytic; /evidence=ECO:0000269|PubMed:11223512; METAL 311; /note=Zinc; catalytic; /evidence=ECO:0000269|PubMed:11223512 |
| Rhea ID | |
| Cross Reference Brenda | 3.4.24.20; |