| IED ID | IndEnz0002006744 |
| Enzyme Type ID | protease006744 |
| Protein Name |
Genome polyprotein Cleaved into: Putative leader protein; Capsid protein 1 CP-1 22.5 kDa protein Coat protein 1 ; Capsid protein 2 CP-2 26 kDa protein Coat protein 2 ; Capsid protein 3 CP-3 31 kDa protein Coat protein 3 ; Putative helicase EC 3.6.4.- Putative NTP-binding protein ; Probable picornain 3C-like protease 3C-like protease EC 3.4.22.- ; Probable RNA-directed RNA polymerase EC 2.7.7.48 |
| Gene Name | |
| Organism | Parsnip yellow fleck virus (isolate P-121) (PYFV) |
| Taxonomic Lineage | Viruses Riboviria Orthornavirae Pisuviricota Pisoniviricetes Picornavirales Secoviridae Sequivirus Parsnip yellow fleck virus Parsnip yellow fleck virus (isolate P-121) (PYFV) |
| Enzyme Sequence | MSSSNSQNSVNMVDGVDLNDPTAVAIAAASGTWGELDRASTCMYNFFDVSDVERNPGESSKQLVSRIKKRAGALLGVSRAFKDTEQVLSAERCAFKSFSDDEEIMATTFEPLKDRAEITPTAASKLDKTLEAHRAKFNYMAIDSIRVAVTSLMHQGDSRECIMYLCDRRFKDPLLGAIALIGFTLPGMQTHVYKTGRMMAFSRKEAIAADRLQLYLYVKGAKLERTQNTPITVNVRTSLIFGSNAENLLKCDSQIDTDMNIVSFMAQNQDLFGWLEAAQGGGYVPQSLRSATTHTSNSVLLHKWNNPVGSVSQRIASRMIFTKAIDAGTSYEDSDVVGSNAKCPNSIGKNLRIGVAQASIQNTKDDSTPYSLADFVQDGPIAPVVASLESFMSSPSISQTLPLINDQFTRPIYSRTFEWKATDTVGASIFQLELPGDVVGPQASSLFSDTMQRAFCFSSDFELSILLTGNESYMGALKIVTDQLRRFHEAKQDDARVFHSMPGRTVFAKDSDGIKIPIEFMSIHKAVSAHDSNSHNALSRVEARVVTPLSHISLSSPVLSITIQVFAKNVKADYMMWRSLETTFPTANATLPSAVGDNFGRLRTSQSEILSTSQILGLLTERAFLGTAKVQQDTGARVIIAEFALHPMSSRNVDGTLLLSQLAALSAMYAFWRGSLVLTFEINCSASTRGKLIVSVTPKGGVALAGITASHQGYGAEFDLGTSSTRSFTMPFVSTDEWESIGDDGIMSAFEGVWDCPVANLLVLHPITSIAESTPSVDIRCYLHPGPDFQLRGRRHIGLRAASRPLSIAQASPVLSQVDFSLMASISIDATEESVVVAVPCAPWYSKEEVDYTLLQNPLHWASRMFTLWRGDIEYRFVVKEEALGDGWQSPISVWHNPNTQLSKCKITKISNKKISKETYHGKKFCLMQLKSIDIVAVDDRRFSWRLCKILDTTKDTAGDSTSPSVTQITYTGHPPMSSQTGVVCIKFPKNSIKGKLKVYSKPGENFEFRHLGGVPSLQVSQMVKYKKPFQNSVPDVFITPSKESSKKELGFKPKVVESAAVPKLAVGQAQGLVSKIKGFGSLWKFDEEEETLNSELQKMAVEVSGEIDPIQDEGWAKRKINEIVSSVSTKLIEASTSILSNAATRAISTLFDVMIGKVRGVLSSLVDSISGAFKMCLGDPKCLCLIGISISAVLGYCTLKLVENSVPDALGIFKALMMVAITSISALYWPKAAISIVTKYEEQFKDIENYCSTIYKHIFLGVSEDKMEGATPAKACATNFEDLAHGKAQAGGKSFLELAGLIAYIRLCVVLCKAMNTSFLEPFTPSNMEKQCRTVGGISIGVKTLCEFKDYIYRMIVGGITPTSSYVKVSGLTGFDIREWFEEVESVTLQETRYTQMGSDEKIKQIRALYDKGVNVMGKLTMIDSPHLSRVCERSFRLCKELLDETHRCKGASSTRVDPFHVSLYGSPGVGKSFVMGKLLDDVLDFMSEPQADRCYSKTPNEEYWSGYIGQTAVKCDDLGQDLSKGFSPTYNQIIQMKTNNCFIVPMADLANKGRTFTSKYIFSTTNVPGCGTKHGLADPGAFMRRRNIFVEVETEGDMIPGSTNHMRFTLLNPLNPDERIMKYPARMKYVDFLCVCVAEARVYFETQNLVMETLNGTTKNQEEPSKDVIAILEELGDGVVEGILEKRKELLSQFGVMDPPPFDAIELEPGKAQASVCFSTDAFGNPLKNPFVELFGKLRDEFERATKQEMPDDILTKFGASLTLGEPTVFGYENQCGMHSAKDSNLMSSFFTFIFGKNLIYKQEQEFLRHIDTLSSMGVLRLVDAVTTTTKGDKKILSFANIYDNQAFEQLGVLERLIFHLVLATRAAKKGRINGIRERLANWFTSARILSNNILEELPSPIKMLLVLATSVGSLYLAFKGLSGIGSMILGFTGNFTAKEEDFEMISLNALMGQAKSKGRNFITSGDELTTRLSRMMSRASLATGRAQGGRSHMDTCEALLARQGQITNMATGLHLVATDLGGGFLLAPLHTFAGAEKDDIFRFQNGADYYFAFEPKDVSQLSEYDACIIRTDAIPMKSSIVSIFAKESQIELLVDMSAHFVCGPWKVPFGGEFISEQTVAKRIASFKYFMDEKLYMAINGWSSPFKTEDGQCGSCLVSTSDKLDGKVFCSLVAGTYDRVTGKYVSTYVPITCDMIKKSISLLTGAEFSESQSSICDSPISDTVAETIKVDQLFSSKPGASGKFGVFGVNDTIGIIDVVGRTFPETTPKSITKSTIVPSLIQPYMPRKPLTEPAILDPRDVRLGENRYDPMIDGIKKYEEQARPIKISWRNQIIESMAAQMQDWETFMVREGYMTMDLPMSVVINGIDGVEYYEPLNMSTSEGYPLILNRPKDAHGKEYLFETMESGERRIKSAKLEAHYESYGHALQSTEPFPLICIECPKDERRALDKIYEKPKTRLFSILPVEFNMHARRLFLDFNVFVMANRHKHGIMVGINPHSREWSDLAISLASFSPYGFNGDFANFDGMFHPSSFSMVSELANIFYGNFLSTERDNLTRMLTNRFSLMKGAILRVPGGGPSGFPMTVIFNSFINLFYLQSAWIMLARFNGRQDISHPCNFPKYVRACVYGDDNIVAIKMEVLPWYNLQTVSEALFDYFGVTMTDGAKNKASEAKPYGKILEFDFLKRHFKADELIPSLFHAPLHKRSIEEQVYWIREGGNSLELLEANIENALYEAHHHGREYYEELKDQIKKAMNRAGYMSFVAPSYLMCRQRWLQQDLGEVATSSLPSHVGLLKEATKNHFSALTGQEEIKAIFEEIDNGNGGTTKHGNMQQILPNIFIGPTRIFETKYGNSLFNLVCDNSLSKGQTRYGVKHGIQSLSKPDFTYISESLPCLTTPNFRMVCLDPIGGELALATALCLLHAAGIINTKTFTMFMRIHIKQWKHVLQAYFRVCETFVSKEWQNFKRDIKRLSQDDVGCSRTTPVCGRFLTLDGQLPQHIKSLDKIDFKKTRRIKIAQDEDFIIQID |
| Enzyme Length | 3027 |
| Uniprot Accession Number | Q05057 |
| Absorption | |
| Active Site | ACT_SITE 2033; /note=For picornain 3C-like protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01222; ACT_SITE 2068; /note=For picornain 3C-like protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01222; ACT_SITE 2155; /note=For picornain 3C-like protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01222 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539}; |
| DNA Binding | |
| EC Number | 3.6.4.-; 3.4.22.-; 2.7.7.48 |
| Enzyme Function | FUNCTION: Picornain 3C-like protease is a thiol protease that probably cleaves the polyprotein. {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 1467..1474; /note=ATP; /evidence=ECO:0000255|PROSITE-ProRule:PRU00551 |
| Features | Active site (3); Chain (7); Domain (3); Natural variant (3); Nucleotide binding (1); Site (3); Transmembrane (3) |
| Keywords | ATP-binding;Capsid protein;Direct protein sequencing;Helicase;Host membrane;Hydrolase;Membrane;Nucleotide-binding;Nucleotidyltransferase;Protease;RNA-directed RNA polymerase;Reference proteome;Thiol protease;Transferase;Transmembrane;Transmembrane helix;Viral RNA replication;Virion |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: [Capsid protein 1]: Virion {ECO:0000305}.; SUBCELLULAR LOCATION: [Capsid protein 2]: Virion {ECO:0000305}.; SUBCELLULAR LOCATION: [Capsid protein 3]: Virion {ECO:0000305}.; SUBCELLULAR LOCATION: [Putative helicase]: Host membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | PTM: Specific enzymatic cleavages by picornain 3C-like protease in vivo yield mature proteins. Picornain 3C-like protease is autocatalytically processed (By similarity). {ECO:0000250}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 336,247 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:21248 |
| Cross Reference Brenda |