| IED ID | IndEnz0002006755 |
| Enzyme Type ID | protease006755 |
| Protein Name |
Putative botulinum-like toxin Wo BoNT/Wo EC 3.4.24.69 |
| Gene Name | WOSG25_110680 |
| Organism | Weissella oryzae (strain DSM 25784 / JCM 18191 / LMG 30913 / SG25) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Lactobacillales Lactobacillaceae Weissella Weissella oryzae Weissella oryzae (strain DSM 25784 / JCM 18191 / LMG 30913 / SG25) |
| Enzyme Sequence | MDVLEMFDVNYESPILESFDSTTQSLNDVHVFMSRIQMSAYDADGEGRIEYRNLKLYEISSGIFISTDRLDTGASGVEDDHEMVDYYSSARLTREFLGESLDSQKSDYFEGIKKVFSFYKNKCNESRYIKEFFEEIQFRNICGFPKQAGTSSTDIFDQFNSVDVLLQDPVTSVWNKKVGSKKANIVIIPPATNLPITEACATAGFQPEGFPKLGSGSFFTVQFDPFFSTRFKAHETDDVALLDPTLTLLHEMTHGLHFQKGIANPVNRSGETPAWATTWGRVTGDNDAFKETPMEELLTFNKHTIDDDIEISDHLKSTYIGFLYNGRNEDDPTESVDGVYQNVSSFLNQYRGFEISSDFQHFIESCYGVKYNQESKKFIVNPRNIKRYVQDGFFIDEAKFARILNIKTRSYYTLMPDNLGVWSYRVDILNRLRETFDEDRGLLSQELDFHTALTPVVSENPALELEVAGMQRMVSLPKIKASYLPSDIKIKNFTGQKISHDTILDTNISGIIISKIKYKSDFVVDESMPRSSLNTTNYNLSPIKGTKFETDIRDKTSVKVTVSEITAPMINHVMKLDNSKVLTERPSLNEDLEETFKNTKDVYIPKTTAMMKLKEGADQTLGAVGFAVWSGQILEDLYNLAQKKEVSIDQIKDDLMSILPFYCAYKNLSAEKYEQAFANATLDAFLIFATDGGGFAGLGITVGAIAINSMYAKAETMEAYDSMFGKYVDQYQNDIKNFTLNAYVQWENNILSRLWNESRLAITGFRNMLKTVKTVMEFDATNQAYSEEDRKIIKAKCEEIFSEFPMLMQTFAKNSMTANLENASKIFNDIVWQKIKEELDQYVIDSKKYFLDSLEEAYNNGSISAESYYKYQTEAREKFVSPREVIDLYIAAHDTVVKRKRYIRRYSRKYDLATDFKGNTVHLNGLGEGTQDIQDLYGNYSVYADKKTVSTQEGHFDQTIKIAKDTNTINKVVLAVSSNNGKEYALNKDEQYTISFWLRMPVPSSSEERRIFSYSAVSGVNKEVEELILQVKNNEFVLATANLLRNSEFVIEPRIALNRWVKITIVNENTRIKVYQNDNLLGLIKDSSRKKPIAQRGTFKFYNYNVDYQLDDISYYNGTISQRDIKYTFKEDHGQFVYDHWGERLQYNKAYYLLSDDNKSAFETVYETKRLKLKSVPGVDIKYLGMNDRVYGYYGGLQFKLVPLDSKNMNNYVRWGDKFTMQSIETTNLSLAIIQDNAYFAPTQLKLISNEGKSEEEIFTFDRNIKLQNAAILVGTGNSKQGPISAYKRGYSGDLWINGARLDGYVTVVNKSNYSNDEIQEKFKWIFVPKDANWVE |
| Enzyme Length | 1336 |
| Uniprot Accession Number | A0A069CUU9 |
| Absorption | |
| Active Site | ACT_SITE 251; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095 |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by EDTA and 1,10-phenanthroline (PubMed:27443638). {ECO:0000269|PubMed:27443638}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Limited hydrolysis of proteins of the neuroexocytosis apparatus, synaptobrevins, SNAP25 or syntaxin. No detected action on small molecule substrates.; EC=3.4.24.69; Evidence={ECO:0000269|PubMed:27443638}; |
| DNA Binding | |
| EC Number | 3.4.24.69 |
| Enzyme Function | FUNCTION: When overexpressed the N-terminus (residues 1-476) cleaves rat synaptobrevin-2/VAMP2 between '89-Trp-|-Trp-90' in vitro. This releases the cytoplasmic domain of VAMP2 from the synaptic vesicle membrane, which would prevent the assembly of the trans-SNARE complex on the membrane and thus prevent vesicle-target membrane fusion and neurotransmitter release (PubMed:27443638). {ECO:0000269|PubMed:27443638}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (18); Chain (1); Helix (14); Metal binding (3); Region (1); Turn (7) |
| Keywords | 3D-structure;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 6RIM; |
| Mapped Pubmed ID | 31111466; |
| Motif | |
| Gene Encoded By | |
| Mass | 153,025 |
| Kinetics | |
| Metal Binding | METAL 250; /note=Zinc; via tele nitrogen; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 254; /note=Zinc; via tele nitrogen; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 296; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:P0DPI0 |
| Rhea ID | |
| Cross Reference Brenda |