| IED ID | IndEnz0002006908 |
| Enzyme Type ID | protease006908 |
| Protein Name |
3-hydroxyacyl- acyl-carrier-protein dehydratase FabZ EC 4.2.1.59 3R -hydroxymyristoyl- acyl-carrier-protein dehydratase 3R -hydroxymyristoyl-ACP dehydrase 17 kDa actomyosin component Beta-hydroxyacyl-ACP dehydratase |
| Gene Name | fabZ sefA yaeA b0180 JW0175 |
| Organism | Escherichia coli (strain K12) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli Escherichia coli (strain K12) |
| Enzyme Sequence | MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNVSVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFKRPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA |
| Enzyme Length | 151 |
| Uniprot Accession Number | P0A6Q6 |
| Absorption | |
| Active Site | ACT_SITE 54; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a (3R)-hydroxyacyl-[ACP] = a (2E)-enoyl-[ACP] + H2O; Xref=Rhea:RHEA:13097, Rhea:RHEA-COMP:9925, Rhea:RHEA-COMP:9945, ChEBI:CHEBI:15377, ChEBI:CHEBI:78784, ChEBI:CHEBI:78827; EC=4.2.1.59; Evidence={ECO:0000269|PubMed:10629181, ECO:0000269|PubMed:7806516, ECO:0000269|PubMed:8910376};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:13098; Evidence={ECO:0000269|PubMed:10629181, ECO:0000269|PubMed:7806516, ECO:0000269|PubMed:8910376}; CATALYTIC ACTIVITY: Reaction=(3R)-hydroxybutanoyl-[ACP] = (2E)-butenoyl-[ACP] + H2O; Xref=Rhea:RHEA:41808, Rhea:RHEA-COMP:9626, Rhea:RHEA-COMP:9627, ChEBI:CHEBI:15377, ChEBI:CHEBI:78451, ChEBI:CHEBI:78453; Evidence={ECO:0000269|PubMed:10629181, ECO:0000269|PubMed:8910376};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41809; Evidence={ECO:0000269|PubMed:10629181, ECO:0000269|PubMed:8910376}; CATALYTIC ACTIVITY: Reaction=(3R)-hydroxyhexanoyl-[ACP] = (2E)-hexenoyl-[ACP] + H2O; Xref=Rhea:RHEA:41828, Rhea:RHEA-COMP:9630, Rhea:RHEA-COMP:9631, ChEBI:CHEBI:15377, ChEBI:CHEBI:78457, ChEBI:CHEBI:78458; Evidence={ECO:0000269|PubMed:8910376};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41829; Evidence={ECO:0000269|PubMed:8910376}; CATALYTIC ACTIVITY: Reaction=(3R)-hydroxyoctanoyl-[ACP] = (2E)-octenoyl-[ACP] + H2O; Xref=Rhea:RHEA:41844, Rhea:RHEA-COMP:9634, Rhea:RHEA-COMP:9635, ChEBI:CHEBI:15377, ChEBI:CHEBI:78461, ChEBI:CHEBI:78462; Evidence={ECO:0000269|PubMed:8910376};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41845; Evidence={ECO:0000269|PubMed:8910376}; CATALYTIC ACTIVITY: Reaction=(3R)-hydroxydecanoyl-[ACP] = (2E)-decenoyl-[ACP] + H2O; Xref=Rhea:RHEA:41860, Rhea:RHEA-COMP:9638, Rhea:RHEA-COMP:9639, ChEBI:CHEBI:15377, ChEBI:CHEBI:78466, ChEBI:CHEBI:78467; Evidence={ECO:0000269|PubMed:8910376};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41861; Evidence={ECO:0000269|PubMed:8910376}; CATALYTIC ACTIVITY: Reaction=(3R)-hydroxydodecanoyl-[ACP] = (2E)-dodecenoyl-[ACP] + H2O; Xref=Rhea:RHEA:41876, Rhea:RHEA-COMP:9642, Rhea:RHEA-COMP:9643, ChEBI:CHEBI:15377, ChEBI:CHEBI:78470, ChEBI:CHEBI:78472; Evidence={ECO:0000269|PubMed:8910376};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41877; Evidence={ECO:0000269|PubMed:8910376}; CATALYTIC ACTIVITY: Reaction=(3R)-hydroxytetradecanoyl-[ACP] = (2E)-tetradecenoyl-[ACP] + H2O; Xref=Rhea:RHEA:41892, Rhea:RHEA-COMP:9646, Rhea:RHEA-COMP:9647, ChEBI:CHEBI:15377, ChEBI:CHEBI:78474, ChEBI:CHEBI:78475; Evidence={ECO:0000269|PubMed:7806516, ECO:0000269|PubMed:8910376};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41893; Evidence={ECO:0000269|PubMed:7806516, ECO:0000269|PubMed:8910376}; CATALYTIC ACTIVITY: Reaction=(3R)-hydroxyhexadecanoyl-[ACP] = (2E)-hexadecenoyl-[ACP] + H2O; Xref=Rhea:RHEA:41908, Rhea:RHEA-COMP:9650, Rhea:RHEA-COMP:9651, ChEBI:CHEBI:15377, ChEBI:CHEBI:78480, ChEBI:CHEBI:78481; Evidence={ECO:0000269|PubMed:8910376};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41909; Evidence={ECO:0000269|PubMed:8910376}; CATALYTIC ACTIVITY: Reaction=(3R)-hydroxy-(9Z)-hexadecenoyl-[ACP] = (2E,9Z)-hexadecadienoyl-[ACP] + H2O; Xref=Rhea:RHEA:54932, Rhea:RHEA-COMP:14036, Rhea:RHEA-COMP:14040, ChEBI:CHEBI:15377, ChEBI:CHEBI:138403, ChEBI:CHEBI:138407; Evidence={ECO:0000269|PubMed:8910376};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:54933; Evidence={ECO:0000269|PubMed:8910376}; CATALYTIC ACTIVITY: Reaction=(3R)-hydroxy-5-methylhexanoyl-[ACP] = (2E)-5-methylhexenoyl-[ACP] + H2O; Xref=Rhea:RHEA:55128, Rhea:RHEA-COMP:14095, Rhea:RHEA-COMP:14097, ChEBI:CHEBI:15377, ChEBI:CHEBI:78986, ChEBI:CHEBI:138610; Evidence={ECO:0000269|PubMed:10629181};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:55129; Evidence={ECO:0000269|PubMed:10629181}; |
| DNA Binding | |
| EC Number | 4.2.1.59 |
| Enzyme Function | FUNCTION: Involved in unsaturated fatty acids biosynthesis. Catalyzes the dehydration of short chain beta-hydroxyacyl-ACPs and long chain saturated and unsaturated beta-hydroxyacyl-ACPs. {ECO:0000269|PubMed:10629181, ECO:0000269|PubMed:7806516, ECO:0000269|PubMed:8910376}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (6); Chain (1); Helix (3); Mutagenesis (1); Turn (1) |
| Keywords | 3D-structure;Cytoplasm;Direct protein sequencing;Lipid A biosynthesis;Lipid biosynthesis;Lipid metabolism;Lyase;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm. |
| Modified Residue | |
| Post Translational Modification | PTM: The N-terminus is blocked. |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 6N3P; |
| Mapped Pubmed ID | 15004283; 15690043; 16606699; 30872475; |
| Motif | |
| Gene Encoded By | |
| Mass | 17,033 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:13097; RHEA:13098; RHEA:41808; RHEA:41809; RHEA:41828; RHEA:41829; RHEA:41844; RHEA:41845; RHEA:41860; RHEA:41861; RHEA:41876; RHEA:41877; RHEA:41892; RHEA:41893; RHEA:41908; RHEA:41909; RHEA:54932; RHEA:54933; RHEA:55128; RHEA:55129 |
| Cross Reference Brenda |