| IED ID | IndEnz0002006915 |
| Enzyme Type ID | protease006915 |
| Protein Name |
Type II secretion system ATPase E T2SS protein E EC 7.4.2.8 Cholera toxin secretion protein EpsE General secretion pathway protein E Type II traffic warden ATPase |
| Gene Name | epsE VC_2732 |
| Organism | Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Vibrionales Vibrionaceae Vibrio Vibrio cholerae Vibrio cholerae O1 Vibrio cholerae O1 biovar El Tor Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) |
| Enzyme Sequence | MTEMVISPAERQSIRRLPFSFANRFKLVLDWNEDFSQASIYYLAPLSMEALVETKRVVKHAFQLIELSQAEFESKLTQVYQRDSSEARQLMEDIGADSDDFFSLAEELPQNEDLLESEDDAPIIKLINAMLGEAIKEGASDIHIETFEKTLSIRFRVDGVLREVLAPSRKLSSLLVSRVKVMAKLDIAEKRVPQDGRISLRIGGRAVDVRVSTMPSSHGERVVMRLLDKNATRLDLHSLGMTAHNHDNFRRLIKRPHGIILVTGPTGSGKSTTLYAGLQELNSSERNILTVEDPIEFDIDGIGQTQVNPRVDMTFARGLRAILRQDPDVVMVGEIRDLETAQIAVQASLTGHLVMSTLHTNTAVGAVTRLRDMGIEPFLISSSLLGVLAQRLVRTLCPDCKEPYEADKEQRKLFDSKKKEPLILYRATGCPKCNHKGYRGRTGIHELLLVDDALQELIHSEAGEQAMEKHIRATTPSIRDDGLDKVRQGITSLEEVMRVTKES |
| Enzyme Length | 503 |
| Uniprot Accession Number | P37093 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=ATP + H(2)O + cellular protein(Side 1) = ADP + phosphate + cellular protein(Side 2).; EC=7.4.2.8; Evidence={ECO:0000269|PubMed:15601709, ECO:0000269|PubMed:27168165}; |
| DNA Binding | |
| EC Number | 7.4.2.8 |
| Enzyme Function | FUNCTION: ATPase component of the type II secretion system required for the energy-dependent secretion of extracellular factors such as proteases and toxins from the periplasm. Acts as a molecular motor to provide the energy that is required for assembly of the pseudopilus and the extrusion of substrates generated in the cytoplasm. {ECO:0000269|PubMed:15601709, ECO:0000269|PubMed:27168165}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Beta strand (21); Chain (1); Helix (21); Metal binding (4); Mutagenesis (5); Turn (2) |
| Keywords | 3D-structure;ATP-binding;Cell inner membrane;Cell membrane;Membrane;Metal-binding;Nucleotide-binding;Protein transport;Reference proteome;Translocase;Transport;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250|UniProtKB:Q00512}. Note=Membrane association is not an intrinsic property but requires the EpsL/GspL gene product. {ECO:0000250|UniProtKB:Q00512}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (5) |
| Cross Reference PDB | 1P9R; 1P9W; 2BH1; 4KSR; 4KSS; |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 56,359 |
| Kinetics | |
| Metal Binding | METAL 397; /note="Zinc"; /evidence="ECO:0007744|PDB:1P9R, ECO:0007744|PDB:1P9W"; METAL 400; /note="Zinc"; /evidence="ECO:0007744|PDB:1P9R, ECO:0007744|PDB:1P9W"; METAL 430; /note="Zinc"; /evidence="ECO:0007744|PDB:1P9R, ECO:0007744|PDB:1P9W"; METAL 433; /note="Zinc"; /evidence="ECO:0007744|PDB:1P9R, ECO:0007744|PDB:1P9W" |
| Rhea ID | |
| Cross Reference Brenda | 7.4.2.8; |