| IED ID | IndEnz0002006947 |
| Enzyme Type ID | protease006947 |
| Protein Name |
Granzyme M EC 3.4.21.- Met-ase Natural killer cell granular protease RNK-Met-1 Fragment |
| Gene Name | Gzmm |
| Organism | Rattus norvegicus (Rat) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Rattus Rattus norvegicus (Rat) |
| Enzyme Sequence | LLLLLALKTLWAVGNRFEAQIIGGREAVPHSRPYMVSLQNTKSHMCGGVLVHQKWVLTAAHCLSEPLQQLKLVFGLHSLHDPQDPGLTFYIKQAIKHPGYNLKYENDLALLKLDGRVKPSKNVKPLALPRKPRDKPAEGSRCSTAGWGITHQRGQLAKSLQELDLRLLDTRMCNNSRFWNGVLTDSMLCLKAGAKGQAPCKGDSGGPLVCGKGKVDGILSFSSKNCTDIFKPTVATAVAPYSSWIRKVIGRWSPQPLT |
| Enzyme Length | 258 |
| Uniprot Accession Number | Q03238 |
| Absorption | |
| Active Site | ACT_SITE 61; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 107; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 204; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Cleaves peptide substrates after methionine, leucine, and norleucine. Physiological substrates include EZR, alpha-tubulins and the apoptosis inhibitor BIRC5/Survivin. Promotes caspase activation and subsequent apoptosis of target cells (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (4); Domain (1); Glycosylation (2); Non-terminal residue (1); Propeptide (1); Region (1); Signal peptide (1) |
| Keywords | Apoptosis;Cytolysis;Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Immunity;Innate immunity;Protease;Reference proteome;Secreted;Serine protease;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. Cytoplasmic granule. Note=Granules of large granular lymphocytes. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL <1..?; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 22206666; |
| Motif | |
| Gene Encoded By | |
| Mass | 28,339 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.4.21.B2; |