| IED ID | IndEnz0002007012 |
| Enzyme Type ID | protease007012 |
| Protein Name |
Serine hydroxymethyltransferase 1, mitochondrial AtSHMT1 EC 2.1.2.1 Glycine hydroxymethyltransferase 1 Serine Transhydroxymethyltransferase STM Serine methylase 1 |
| Gene Name | SHM1 SHMT1 STM At4g37930 F20D10.50 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MAMAMALRRLSSSIDKPIRPLIRSTSCYMSSLPSEAVDEKERSRVTWPKQLNAPLEEVDPEIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMAETLCQKRALEAFRLDPEKWGVNVQPLSGSPANFHVYTALLKPHERIMALDLPHGGHLSHGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQMEKSATLFRPKLIVAGASAYARLYDYARIRKVCNKQKAVMLADMAHISGLVAANVIPSPFDYADVVTTTTHKSLRGPRGAMIFFRKGVKEINKQGKEVLYDFEDKINQAVFPGLQGGPHNHTITGLAVALKQATTSEYKAYQEQVLSNSAKFAQTLMERGYELVSGGTDNHLVLVNLKPKGIDGSRVEKVLEAVHIASNKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEYFDKAVTIALKVKSEAQGTKLKDFVSAMESSSTIQSEIAKLRHEVEEFAKQFPTIGFEKETMKYKN |
| Enzyme Length | 517 |
| Uniprot Accession Number | Q9SZJ5 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine; Xref=Rhea:RHEA:15481, ChEBI:CHEBI:15377, ChEBI:CHEBI:15636, ChEBI:CHEBI:33384, ChEBI:CHEBI:57305, ChEBI:CHEBI:57453; EC=2.1.2.1; Evidence={ECO:0000269|PubMed:21976482}; |
| DNA Binding | |
| EC Number | 2.1.2.1 |
| Enzyme Function | FUNCTION: Functions in the photorespiratory pathway in catalyzing the interconversion of serine and glycine. Involved in controlling cell damage caused by abiotic stress, such as high light and salt and the hypersensitive defense response of plants. {ECO:0000269|PubMed:15659103, ECO:0000269|PubMed:16339799, ECO:0000269|PubMed:21976482, ECO:0000269|PubMed:23232097}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion. {ECO:0000305}. |
| nucleotide Binding | |
| Features | Chain (1); Modified residue (1); Sequence conflict (1); Transit peptide (1) |
| Keywords | Cytoplasm;Mitochondrion;One-carbon metabolism;Pyridoxal phosphate;Reference proteome;Transferase;Transit peptide;Ubl conjugation |
| Interact With | Q9ZNZ7; Q9SB51 |
| Induction | INDUCTION: Circadian-regulation. Induction by light. {ECO:0000269|PubMed:10806255}. |
| Subcellular Location | SUBCELLULAR LOCATION: Mitochondrion {ECO:0000305|PubMed:25862457}. Cytoplasm. |
| Modified Residue | MOD_RES 286; /note=N6-(pyridoxal phosphate)lysine; /evidence=ECO:0000250|UniProtKB:P34896 |
| Post Translational Modification | PTM: Ubiquitinated. {ECO:0000269|PubMed:23232097}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 14617066; 15028209; 15821981; 16336779; 16502469; 16923014; 17317660; 17400706; 17432890; 17447911; 17511964; 18385124; 18538804; 18650403; 18775970; 19175771; 19704719; 20118269; 21223391; 23551974; 23800877; 23857362; 25274430; 25641188; 26098229; 26116608; 26220906; 26380567; 27170143; 28627464; 31134633; 32344669; |
| Motif | |
| Gene Encoded By | |
| Mass | 57,401 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:15481 |
| Cross Reference Brenda |