| IED ID | IndEnz0002007037 |
| Enzyme Type ID | protease007037 |
| Protein Name |
Iron-regulated surface determinant protein A Fur-regulated protein A Staphylococcal transferrin-binding protein A |
| Gene Name | isdA frpA stbA NWMN_1041 |
| Organism | Staphylococcus aureus (strain Newman) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Staphylococcaceae Staphylococcus Staphylococcus aureus Staphylococcus aureus (strain Newman) |
| Enzyme Sequence | MTKHYLNSKYQSEQRSSAMKKITMGTASIILGSLVYIGADSQQVNAATEATNATNNQSTQVSQATSQPINFQVQKDGSSEKSHMDDYMQHPGKVIKQNNKYYFQTVLNNASFWKEYKFYNANNQELATTVVNDNKKADTRTINVAVEPGYKSLTTKVHIVVPQINYNHRYTTHLEFEKAIPTLADAAKPNNVKPVQPKPAQPKTPTEQTKPVQPKVEKVKPTVTTTSKVEDNHSTKVVSTDTTKDQTKTQTAHTVKTAQTAQEQNKVQTPVKDVATAKSESNNQAVSDNKSQQTNKVTKHNETPKQASKAKELPKTGLTSVDNFISTVAFATLALLGSLSLLLFKRKESK |
| Enzyme Length | 350 |
| Uniprot Accession Number | A6QG31 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | BINDING 75; /note=Heme; /evidence=ECO:0000250; BINDING 82; /note=Heme; /evidence=ECO:0000250 |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Cell wall-anchored surface receptor that participates in the extraction of heme from oxidized methemoglobin/metHb to enable growth on hemoglobin as a sole iron source (PubMed:17229211, PubMed:12574635). Receives heme from IsdB and transfers it to IsdC (By similarity). Plays also a role in the inhibition of host immune response (PubMed:18097052, PubMed:18005699, PubMed:18227165). Protects S.aureus against the bactericidal protease activity of apolactoferrin (PubMed:18097052). Enhances bacterial cellular hydrophobicity, which renders S.aureus resistant to bactericidal human skin fatty acids as well as to beta-defensins and cathelicidin (PubMed:18005699). Also binds fibronectin and chains B-beta and gamma of fibrinogen, promoting clumping of S.aureus with fibrinogen. Involved in adherence of S.aureus to human desquamated nasal epithelial cells and is required for nasal colonization. {ECO:0000250|UniProtKB:Q7A152, ECO:0000269|PubMed:12574635, ECO:0000269|PubMed:17229211, ECO:0000269|PubMed:18005699, ECO:0000269|PubMed:18097052, ECO:0000269|PubMed:18227165}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Binding site (2); Chain (1); Compositional bias (2); Domain (1); Metal binding (1); Modified residue (1); Motif (1); Propeptide (1); Region (1); Signal peptide (1) |
| Keywords | Cell wall;Heme;Iron;Metal-binding;Peptidoglycan-anchor;Secreted;Signal |
| Interact With | |
| Induction | INDUCTION: Repressed by fur in the presence of iron. |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000269|PubMed:19398548}; Peptidoglycan-anchor {ECO:0000305}. Note=Encodes an LPXTG motif-containing sorting signal that targets to the cell wall, which is catalyzed by sortase A. {ECO:0000305|PubMed:12574635}. |
| Modified Residue | MOD_RES 316; /note=Pentaglycyl murein peptidoglycan amidated threonine; /evidence=ECO:0000255|PROSITE-ProRule:PRU00477 |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..46; /evidence=ECO:0000250 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 313..317; /note=LPXTG sorting signal; /evidence=ECO:0000255|PROSITE-ProRule:PRU00477 |
| Gene Encoded By | |
| Mass | 38,746 |
| Kinetics | |
| Metal Binding | METAL 166; /note=Iron (heme axial ligand); /evidence=ECO:0000250|UniProtKB:Q7A655 |
| Rhea ID | |
| Cross Reference Brenda |