| IED ID | IndEnz0002007160 |
| Enzyme Type ID | protease007160 |
| Protein Name |
Myofibril-bound serine protease MBSP EC 3.4.21.- Fragment |
| Gene Name | |
| Organism | Saurida wanieso (Wanieso lizardfish) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Actinopterygii Actinopteri Neopterygii Teleostei Osteoglossocephalai Clupeocephala Euteleosteomorpha Neoteleostei Eurypterygia Aulopa Aulopiformes (grinners and others) Aulopoidei Synodontidae Harpadontinae (Bombay ducks) Saurida Saurida wanieso (Wanieso lizardfish) |
| Enzyme Sequence | IVGGAECVPY |
| Enzyme Length | 10 |
| Uniprot Accession Number | P84476 |
| Absorption | |
| Active Site | |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by the serine protease inhibitors Pefabloc SC, STI, aprotinin, benzamidine, leupeptin and antipain, and by the cysteine proteinase inhibitors DTNB and pCMB. Not inhibited by the cysteine protease inhibitor E-64, the aspartic proteinase inhibitor pepstatin A and the metalloproteinase inhibitor EDTA. {ECO:0000269|PubMed:10817913}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Serine protease which degrades the myosin heavy chain but not actin or alpha-actinin. Selectively cleaves Arg-|-Xaa bonds. {ECO:0000269|PubMed:10817913}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 55 degrees Celsius. Active from 30 to 65 degrees Celsius. {ECO:0000269|PubMed:10817913}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 8.0. Active from pH 5.0 to 11.0. {ECO:0000269|PubMed:10817913}; |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Domain (1); Non-terminal residue (1) |
| Keywords | Cytoplasm;Direct protein sequencing;Hydrolase;Protease;Serine protease |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:10817913}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 1,007 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |