| IED ID | IndEnz0002007293 |
| Enzyme Type ID | protease007293 |
| Protein Name |
Serralysin EC 3.4.24.40 Secreted alkaline metalloproteinase |
| Gene Name | prtA1 |
| Organism | Photorhabdus luminescens (Xenorhabdus luminescens) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Morganellaceae Photorhabdus Photorhabdus luminescens (Xenorhabdus luminescens) |
| Enzyme Sequence | MEKYMSLKKKISYSEDKDVSGSEKANELLKWLQAYIPGKDSNIVVEHEPSKDAAKELIRGDYRWGHQDDDKSKAFQLKYTFLESKPDDMPWHISEFSAFNEKQKAAAKLSIQSWADVANINFVETTDAKEANITFGFFDVSLTGSYAFAYLPRPEKTQLGTWYNAKSRTFSNNDIDVNGYGRQTFTHEIGHTLGLQHPADYNASDEVSPTYKNSATYFEDSRAYTVMSYFSEKNTGQDFKGIYSSAPLLNDISAIQAVYGANNTIRADDTVYGFNSNTDRDFYTAKDENSKLLFTAWDTGGNDTFDFSGFTQDQRINLNEASFSDVGGLKGNVSIARGVTIENAIGGSGNDVLIGNDAANTLKGGAGDDIIYGGLGADNLWGGEGKDTFVYLSAKESPPLERDWIHDFVSGEDKIDVSLFDLGEAGKGGVRFVREFTGEVGEAVLRYNTVDKVNDFAINLGGEFSYDDFWVKIVGEPILESDFILS |
| Enzyme Length | 486 |
| Uniprot Accession Number | Q84F70 |
| Absorption | |
| Active Site | ACT_SITE 188; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Preferential cleavage of bonds with hydrophobic residues in P1'.; EC=3.4.24.40; |
| DNA Binding | |
| EC Number | 3.4.24.40 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Metal binding (12); Repeat (2) |
| Keywords | Calcium;Hydrolase;Metal-binding;Metalloprotease;Protease;Repeat;Secreted;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 53,860 |
| Kinetics | |
| Metal Binding | METAL 187; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 191; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 197; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 266; /note=Calcium 1; via carbonyl oxygen; /evidence=ECO:0000250; METAL 269; /note=Calcium 1; /evidence=ECO:0000250; METAL 298; /note=Calcium 1; /evidence=ECO:0000250; METAL 300; /note=Calcium 1; via carbonyl oxygen; /evidence=ECO:0000250; METAL 301; /note=Calcium 2; via carbonyl oxygen; /evidence=ECO:0000250; METAL 303; /note=Calcium 1; /evidence=ECO:0000250; METAL 303; /note=Calcium 2; /evidence=ECO:0000250; METAL 340; /note=Calcium 2; via carbonyl oxygen; /evidence=ECO:0000250; METAL 342; /note=Calcium 2; /evidence=ECO:0000250 |
| Rhea ID | |
| Cross Reference Brenda |