| IED ID | IndEnz0002007316 |
| Enzyme Type ID | protease007316 |
| Protein Name |
Lon protease homolog 2, peroxisomal EC 3.4.21.53 |
| Gene Name | AN0122 |
| Organism | Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Nidulantes Emericella nidulans (Aspergillus nidulans) Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) |
| Enzyme Sequence | MGTNGGRPTKLSLVPLPKGSVLLPGATLRIPVSNRPDLANLLSSLLDRTNAIRRDANSITFGCVPLCSPYLSKDGQHVIDNGTVDEDKKEEFESLEAGQARKEDLYRYGTLGKVIGVQRRAYSEPHLLVQGVQRLTVRRVLRERPFFEAECILHDEKETPLNDRETAELFQQLRQLSRELLTLLRYTSLIPNTGGPRLSPLIARKFELIITKSDLAQAGRLADVMADIAESGLEDKLRVLAAFDVKTRLERVVDILNKQNQIIRGSVKFTTISTDNIPPASVLDISQIDPRIRDLLSRRGIPGASGTPPPGLGGRNNEADEKESNELDELQQRLKDAQLSPEAQKVADKEMRRLRKMMPVNQEYGVIRTYLENLADIPWTKVTEDKLGPETLKAARKQLDDDHYGLEKIKKRLLEYLAVLRLKQSTNQGLEQQISILTKELDNSGGDIEKDIPSLPESDRAAIESKLNALTSKRTVDKSPILLLVGPPGTGKTSLARSVATALGRKFHRISLGGVRDEAEIRGHRRTYVAAMPGVIVNGLKKVGVANPVFLLDEIDKIGGPNFQGDPSAAMLEVLDPEQNHTFVDHYINIPIDLSKVLFIATANSLDTIPAPLLDRMETIQLSGYTTVEKRHIAKRHLLPKQIRANGLSDGQVVLSDDVIDKTTTSYTRESGVRNLERELGSICRYKAVQFADATDSAKLESYNPVVTVDDLEEILGIERFDEEIAEKHGRPGVVTGLVAYSTGGQGSILFIEVADMPGSGRVQLTGKLGDVLKESVEVALTWVKAHSFELGLTSDPNEDIMKNRSLHVHCPSGAIPKDGPSAGLAHTIGLISLFSGKAVPPKLAMTGEVSLRGRVMPVGGIKEKLIGALRAGVTTVLLPHQNRKDVKDVPEEVSNGLEIIYVKHIWEAIRHIWPDAHWPGQHHMDFVESRL |
| Enzyme Length | 932 |
| Uniprot Accession Number | Q5BH58 |
| Absorption | |
| Active Site | ACT_SITE 822; /evidence=ECO:0000255|HAMAP-Rule:MF_03121; ACT_SITE 865; /evidence=ECO:0000255|HAMAP-Rule:MF_03121 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of proteins in presence of ATP.; EC=3.4.21.53; Evidence={ECO:0000255|HAMAP-Rule:MF_03121}; |
| DNA Binding | |
| EC Number | 3.4.21.53 |
| Enzyme Function | FUNCTION: ATP-dependent serine protease that mediates the selective degradation of misfolded and unassembled polypeptides in the peroxisomal matrix. Necessary for type 2 peroxisome targeting signal (PTS2)-containing protein processing and facilitates peroxisome matrix protein import. {ECO:0000255|HAMAP-Rule:MF_03121}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 486..493; /note=ATP; /evidence=ECO:0000255|HAMAP-Rule:MF_03121 |
| Features | Active site (2); Chain (1); Domain (2); Motif (1); Nucleotide binding (1); Region (1) |
| Keywords | ATP-binding;Hydrolase;Nucleotide-binding;Peroxisome;Protease;Reference proteome;Serine protease |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Peroxisome matrix {ECO:0000255|HAMAP-Rule:MF_03121}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 930..932; /note=Microbody targeting signal; /evidence=ECO:0000255|HAMAP-Rule:MF_03121 |
| Gene Encoded By | |
| Mass | 102,748 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |