| IED ID | IndEnz0002007332 |
| Enzyme Type ID | protease007332 |
| Protein Name |
Lysostaphin EC 3.4.24.75 Glycyl-glycine endopeptidase |
| Gene Name | lss |
| Organism | Staphylococcus simulans |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Staphylococcaceae Staphylococcus Staphylococcus simulans |
| Enzyme Sequence | MKKTKNNYYTRPLAIGLSTFALASIVYGGIQNETHASEKSNMDVSKKVAEVETSKAPVENTAEVETSKAPVENTAEVETSKAPVENTAEVETSKAPVENTAEVETSKAPVENTAEVETSKAPVENTAEVETSKAPVENTAEVETSKAPVENTAEVETSKAPVENTAEVETSKAPVENTAEVETSKAPVENTAEVETSKAPVENTAEVETSKAPVENTAEVETSKAPVENTAEVETSKALVQNRTALRAATHEHSAQWLNNYKKGYGYGPYPLGINGGMHYGVDFFMNIGTPVKAISSGKIVEAGWSNYGGGNQIGLIENDGVHRQWYMHLSKYNVKVGDYVKAGQIIGWSGSTGYSTAPHLHFQRMVNSFSNSTAQDPMPFLKSAGYGKAGGTVTPTPNTGWKTNKYGTLYKSESASFTPNTDIITRTTGPFRSMPQSGVLKAGQTIHYDEVMKQDGHVWVGYTGNSGQRIYLPVRTWNKSTNTLGVLWGTIK |
| Enzyme Length | 493 |
| Uniprot Accession Number | P10547 |
| Absorption | |
| Active Site | ACT_SITE 360 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of the -Gly-|-Gly- bond in the pentaglycine inter-peptide link joining staphylococcal cell wall peptidoglycans.; EC=3.4.24.75; |
| DNA Binding | |
| EC Number | 3.4.24.75 |
| Enzyme Function | FUNCTION: Lyses staphylococcal cells by hydrolyzing the polyglycine interpeptide bridges of the peptidoglycan. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (21); Chain (1); Compositional bias (1); Domain (1); Helix (3); Metal binding (3); Propeptide (1); Region (2); Repeat (15); Sequence conflict (1); Signal peptide (1); Turn (3) |
| Keywords | 3D-structure;Cell wall biogenesis/degradation;Hydrolase;Metal-binding;Metalloprotease;Protease;Repeat;Secreted;Signal;Zinc;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..23; /evidence=ECO:0000255 |
| Structure 3D | NMR spectroscopy (1); X-ray crystallography (6) |
| Cross Reference PDB | 4LXC; 4QP5; 4QPB; 5LEO; 5NMY; 6RJE; 6RK4; |
| Mapped Pubmed ID | 25039253; 30018958; 31686030; |
| Motif | |
| Gene Encoded By | |
| Mass | 53,091 |
| Kinetics | |
| Metal Binding | METAL 279; /note=Zinc; METAL 283; /note=Zinc; METAL 362; /note=Zinc |
| Rhea ID | |
| Cross Reference Brenda | 3.4.24.75; |