| IED ID | IndEnz0002007334 |
| Enzyme Type ID | protease007334 |
| Protein Name |
Metacaspase-5 EC 3.4.22.- TbMCA5 |
| Gene Name | MCA5 |
| Organism | Trypanosoma brucei brucei |
| Taxonomic Lineage | cellular organisms Eukaryota Discoba Euglenozoa Kinetoplastea (kinetoplasts) Metakinetoplastina Trypanosomatida Trypanosomatidae Trypanosoma Trypanozoon Trypanosoma brucei Trypanosoma brucei brucei |
| Enzyme Sequence | MDAALALLFGQVATAVLPYVVNSIGRVPRPKRVDVKKAMGEAHQCRPVVPYRAPRPYTEGRVKALFIGINYTGSSAQLGGCVNDVMHMLQTLQRIEFPISECCILVDDRRFPNFTAMPTRENIIKYMAWLVYDVRPGDVLFFHFSGHGAETKGGRDSNEKMDQCLVPLDYDKAGAILDDDLFELMIKGLPAGVRMTAVFDCCHSASLLDLPFAFVAGRNVSSNQRHEMRMVRKDNYSRGDVVMFSGCEDSGTSADVTNTSSFGNGTVAAGGAATQAFTWALLNTTGYSYIDIFMKTREVLRQKGYKQVPQLSSSKPVDLYKQFSLFGPLTMNASLVQHLPQEYVQPWAPHPAYQQPHEATLPASVSQPHSQPVMGIPVASTSNGKSNPGVSDGGRASGEVYPPTQYPSSHPAPQQQAYYQPPQQAYYQPPQQAYYQPPQQAYYQPPQQAYYQPPQQAYYQPEPHHQPAPPPPPKKENKPARPGYPMSYCMKFSQGKPGRK |
| Enzyme Length | 500 |
| Uniprot Accession Number | Q8IEW1 |
| Absorption | |
| Active Site | ACT_SITE 147; /evidence=ECO:0000250|UniProtKB:Q2VLK8; ACT_SITE 202; /evidence=ECO:0000250|UniProtKB:Q2VLK8 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.22.- |
| Enzyme Function | FUNCTION: Cysteine protease that cleaves specifically after arginine or lysine residues. {ECO:0000250|UniProtKB:Q2VLK8}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Compositional bias (2); Glycosylation (8); Metal binding (4); Region (3); Sequence conflict (2); Signal peptide (1) |
| Keywords | Calcium;Endosome;Glycoprotein;Hydrolase;Metal-binding;Protease;Signal;Thiol protease |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Recycling endosome {ECO:0000269|PubMed:16507595}. Note=Localizes to RAB11-positive recycling endosomes. {ECO:0000269|PubMed:16507595}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..18; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 55,443 |
| Kinetics | |
| Metal Binding | METAL 162; /note=Calcium; /evidence=ECO:0000250|UniProtKB:Q585F3; METAL 178; /note=Calcium; /evidence=ECO:0000250|UniProtKB:Q585F3; METAL 179; /note=Calcium; /evidence=ECO:0000250|UniProtKB:Q585F3; METAL 209; /note=Calcium; /evidence=ECO:0000250|UniProtKB:Q585F3 |
| Rhea ID | |
| Cross Reference Brenda |