| IED ID | IndEnz0002007342 |
| Enzyme Type ID | protease007342 |
| Protein Name |
Mitochondrial-processing peptidase subunit beta EC 3.4.24.64 Beta-MPP Complex III subunit I Core protein I Cytochrome b-c1 complex subunit 1, mitochondrial Processing enhancing protein Ubiquinol-cytochrome c oxidoreductase core protein 1 Ubiquinol-cytochrome c reductase complex 50 kDa protein |
| Gene Name | pep NCU02549 |
| Organism | Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Sordariomycetidae Sordariales Sordariaceae Neurospora Neurospora crassa Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) |
| Enzyme Sequence | MASRRLALNLAQGVKARAGGVINPFRRGLATPHSGTGIKTQTTTLKNGLTVASQYSPYAQTSTVGMWIDAGSRAETDETNGTAHFLEHLAFKGTTKRTQQQLELEIENMGAHLNAYTSRENTVYFAKALNEDVPKCVDILQDILQNSKLEESAIERERDVILRESEEVEKQLEEVVFDHLHATAYQHQPLGRTILGPRENIRDITRTELVNYIKNNYTADRMVLVGAGGVPHEQLVEMADKYFSKLPATAPVSSASILSKKKPDFIGSDIRIRDDTIPTANIAIAVEGVSWSDDDYFTGLVTQAIVGNYDKALGNAPHQGSKLSGFVHKHDLATSFMSFSTSYSDTGLWGIYLVTDKLDRVDDLVHFSLREWTRLCSNVSEAEVERAKAQLKASILLSLDGTTAVAEDIGRQIVTTGRRMSPAEIERIIDAVSAKDVMDFANKKIWDQDIAISAVGSIEGLFDYARIRGDMSRNAF |
| Enzyme Length | 476 |
| Uniprot Accession Number | P11913 |
| Absorption | |
| Active Site | ACT_SITE 87; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10096 |
| Activity Regulation | ACTIVITY REGULATION: Binding to mpp is required for catalytic activity (PubMed:2967109). Inhibited by metal chelator ethylenediaminetetraacetic acid (EDTA) (PubMed:2967109). {ECO:0000269|PubMed:2967109}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Release of N-terminal transit peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2.; EC=3.4.24.64; Evidence={ECO:0000269|PubMed:2967109, ECO:0000269|PubMed:8106471}; |
| DNA Binding | |
| EC Number | 3.4.24.64 |
| Enzyme Function | FUNCTION: Catalytic subunit of the essential mitochondrial processing protease (MPP), which cleaves the mitochondrial sequence off newly imported precursors proteins (PubMed:2967109). Preferentially, cleaves after an arginine at position P2 (PubMed:8106471). {ECO:0000269|PubMed:2967109, ECO:0000269|PubMed:8106471}.; FUNCTION: Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c. {ECO:0000269|PubMed:1847681, ECO:0000269|PubMed:19239619, ECO:0000269|PubMed:3015618, ECO:0000305|PubMed:2524007}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Metal binding (3); Transit peptide (1) |
| Keywords | Direct protein sequencing;Electron transport;Hydrolase;Membrane;Metal-binding;Metalloprotease;Mitochondrion;Mitochondrion inner membrane;Protease;Reference proteome;Respiratory chain;Transit peptide;Transport;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Mitochondrion matrix {ECO:0000269|PubMed:2967109}. Mitochondrion inner membrane {ECO:0000269|PubMed:226365}; Peripheral membrane protein {ECO:0000250|UniProtKB:P07256}; Matrix side {ECO:0000250|UniProtKB:P07256}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 52,556 |
| Kinetics | |
| Metal Binding | METAL 84; /note=Zinc; via tele nitrogen; /evidence=ECO:0000255|PROSITE-ProRule:PRU10096; METAL 88; /note=Zinc; via tele nitrogen; /evidence=ECO:0000255|PROSITE-ProRule:PRU10096; METAL 164; /note=Zinc; /evidence=ECO:0000255|PROSITE-ProRule:PRU10096 |
| Rhea ID | |
| Cross Reference Brenda |