| IED ID | IndEnz0002007353 |
| Enzyme Type ID | protease007353 |
| Protein Name |
Zinc protease PqqE EC 3.4.24.- |
| Gene Name | pqqE HP_1012 |
| Organism | Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria delta/epsilon subdivisions Epsilonproteobacteria Campylobacterales Helicobacteraceae Helicobacter Helicobacter pylori (Campylobacter pylori) Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) |
| Enzyme Sequence | MKHFSVKRLLGLSSVLLVTLGASMHAQSYLPKHESVTLKNGLQVVSVPLENKTGVIEVDVLYKVGSRNETMGKSGIAHMLEHLNFKSTKNLKAGEFDKIVKRFGGVSNASTSFDITRYFIKTSQANLDKSLELFAETMGSLNLKEDEFLPERQVVAEERRWRTDNSPIGMLYFRFFNTAYVYHPYHWTPIGFMDDIQNWTLKDIKKFHSLYYQPKNAIVLVVGDVNSQKVFELSKKHFESLKNLDEKAIPTPYMKEPKQDGARTAVVHKDGVHLEWVALGYKVPAFKHKDQVALDALSRLLGEGKSSWLQSELVDKKRLASQAFSHNMQLQDESVFLFIAGGNPNVKAEALQKEIVALLEKLKKGEITQAELDKLKINQKADFISNLESSSDVAGLFADYLVQNDIQGLTDYQRQFLDLKVSDLVRVANEYFKDTQSTTVFLKP |
| Enzyme Length | 444 |
| Uniprot Accession Number | O25656 |
| Absorption | |
| Active Site | ACT_SITE 81; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10096 |
| Activity Regulation | ACTIVITY REGULATION: Can function alone, but full activity requires the presence of the non-peptidase homolog YmxG. {ECO:0000269|PubMed:33970782}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.24.- |
| Enzyme Function | FUNCTION: Virulence factor that cleaves the cytoplasmic domain of the human junctional adhesion molecule A (JAM-A), compromising gastric epithelial barrier function and cell-cell adhesion (PubMed:33970782). Cleavage of JAM-A occurs after Ala-285 or, to a lesser extent, before Ala-285 (PubMed:33970782). {ECO:0000269|PubMed:33970782}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Metal binding (3); Mutagenesis (2); Signal peptide (1) |
| Keywords | Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Secreted;Signal;Virulence;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:17533641}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..28; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11196647; |
| Motif | |
| Gene Encoded By | |
| Mass | 50,331 |
| Kinetics | |
| Metal Binding | METAL 78; /note=Zinc; /evidence=ECO:0000255|PROSITE-ProRule:PRU10096; METAL 82; /note=Zinc; /evidence=ECO:0000255|PROSITE-ProRule:PRU10096; METAL 158; /note=Zinc; /evidence=ECO:0000255|PROSITE-ProRule:PRU10096 |
| Rhea ID | |
| Cross Reference Brenda |