| IED ID | IndEnz0002007381 |
| Enzyme Type ID | protease007381 |
| Protein Name |
Pol polyprotein Cleaved into: Protease EC 3.4.23.- Retropepsin ; Reverse transcriptase/ribonuclease H RT EC 2.7.7.49 EC 3.1.26.13 Exoribonuclease H EC 3.1.13.2 ; Deoxyuridine 5'-triphosphate nucleotidohydrolase dUTPase EC 3.6.1.23 ; Integrase IN EC 2.7.7.- EC 3.1.-.- |
| Gene Name | pol |
| Organism | Feline immunodeficiency virus (isolate TM2) (FIV) |
| Taxonomic Lineage | Viruses Riboviria Pararnavirae Artverviricota Revtraviricetes Ortervirales Retroviridae Orthoretrovirinae Lentivirus Feline immunodeficiency virus Feline immunodeficiency virus (isolate TM2) (FIV) |
| Enzyme Sequence | ENLGKREGGASCSPSKPSAANSTICTSNGGETIRFINYNTIGTTTTLERRPEIQIFVNGHPIKFLLDTGADITILNRKDFQIGNSIENGKQNMIGVGGGKRGTNYINVHLEIRDENYRMQCIFGNVCVLEDNSLIQPLLGRDNMIKFNIRLVMAQISEKIPIVKVRMKDPTQGPQVKQWPLSNEKIEALTDIVERLESEGKVKRADPNNPWNTPVFAIKKKSGKWRMLIDFRVLNKLTDKGAEVQLGLPHPAGLQMKKQVTVLDIGDAYFTIPLDPDYAPYTAFTLPRKNNAGPGRRYVWCSLPQGWVLSPLIYQSTLNNILQPFIKQNSELDIYQYMDDIYIGSNLNKKEHKQKVEELRKLLLWWGFETPEDKLQEEPPYKWMGYELHPLTWSIQQKQLEIPERPTLNELQKLAGKINWASQTIPDLSIKELTNMMRGDQKLDSIREWTVEAKREVQKAKEAIETQAQLNYYDPNRGLYAKLSLVGPHQICYQVYQKNPEHILWYGKINRQKKKAENTCDIALRACYKIREESIIRIGKEPVYEIPASREAWESNLIRSPYLKAPPPEVEFIHAALSIKRALSMIQDAPIIGAETWYIDGSRKQGKAARAAYWTNTGKWQIMEIEGSNQKAEVQALLLALKAGSEEMNIITDSQYILNILNQQPDLMEGLWQEVLEQMEKKIAIFIDWVPGHKGIPGNEEVDKLCQTMMIIEGEGILEKRSEDAGYDLLAAAQETHFLPGEVRIVPTKTRIMLPKGHWGLIMGKSSIGSKGVDVLGGVIDEGYRGELGVIMINLTKKSITILEKQKIAQLIILPCRHEGLQQGEIQMNSERGEKGFGSAGVFSSWVDRIEEAELNHEKFHSDPQYLRTEFNLPRIVAEEIKRKCPLCRIRGEQVGGQLKIRPGIWQMDCTHFNGKIIIVAVHVESGFLWAQIIPQETADCTVKALLQLICAHNVTELQTDNGPNFKNQKMEGLLNYMGIKHKLGIPGNPQSQALVENANNTLKVWIQKFLPETTSLDNALALALHCLNFKQRGRLGRMAPYELYIQQESLRIQDYFSAIPQKLMMQWLYYKDQKDKKWKGPMRVEYWGQGSVLLKDEEKGIFLVPRRHIRRVPEPCTLPEGDE |
| Enzyme Length | 1124 |
| Uniprot Accession Number | P31822 |
| Absorption | |
| Active Site | ACT_SITE 67; /note=For protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU10094 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.; EC=3.1.26.13; CATALYTIC ACTIVITY: Reaction=3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid.; EC=3.1.13.2; CATALYTIC ACTIVITY: Reaction=dUTP + H2O = diphosphate + dUMP + H(+); Xref=Rhea:RHEA:10248, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, ChEBI:CHEBI:61555, ChEBI:CHEBI:246422; EC=3.6.1.23; CATALYTIC ACTIVITY: Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339, Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560, ChEBI:CHEBI:173112; EC=2.7.7.49; Evidence={ECO:0000255|PROSITE-ProRule:PRU00405}; |
| DNA Binding | DNA_BIND 1067..1115; /note=Integrase-type; /evidence=ECO:0000255|PROSITE-ProRule:PRU00506 |
| EC Number | 3.4.23.-; 2.7.7.49; 3.1.26.13; 3.1.13.2; 3.6.1.23; 2.7.7.-; 3.1.-.- |
| Enzyme Function | FUNCTION: During replicative cycle of retroviruses, the reverse-transcribed viral DNA is integrated into the host chromosome by the viral integrase enzyme. RNase H activity is associated with the reverse transcriptase. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (4); DNA binding (1); Domain (4); Metal binding (9); Region (1); Zinc finger (1) |
| Keywords | Aspartyl protease;DNA integration;DNA recombination;DNA-binding;Endonuclease;Hydrolase;Metal-binding;Multifunctional enzyme;Nuclease;Nucleotide metabolism;Nucleotidyltransferase;Protease;RNA-directed DNA polymerase;Transferase;Viral genome integration;Virus entry into host cell;Zinc;Zinc-finger |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | PTM: Cleavage sites that yield the mature proteins remain to be determined. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 128,101 |
| Kinetics | |
| Metal Binding | METAL 600; /note=Magnesium 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00408; METAL 600; /note=Magnesium 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00408; METAL 633; /note=Magnesium 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00408; METAL 653; /note=Magnesium 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00408; METAL 703; /note=Magnesium 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00408; METAL 857; /note=Zinc; /evidence=ECO:0000255|PROSITE-ProRule:PRU00450; METAL 861; /note=Zinc; /evidence=ECO:0000255|PROSITE-ProRule:PRU00450; METAL 885; /note=Zinc; /evidence=ECO:0000255|PROSITE-ProRule:PRU00450; METAL 888; /note=Zinc; /evidence=ECO:0000255|PROSITE-ProRule:PRU00450 |
| Rhea ID | RHEA:10248; RHEA:22508 |
| Cross Reference Brenda |