| IED ID | IndEnz0002007420 |
| Enzyme Type ID | protease007420 |
| Protein Name |
Protein P1 66.2 kDa protein Genome-linked protein precursor Protein ORF1 Cleaved into: Serine protease EC 3.4.21.- ; VPg/P1-C25 |
| Gene Name | ORF1 |
| Organism | Turnip yellows virus (isolate FL-1) (TuYV) (BWYV-FL1) |
| Taxonomic Lineage | Viruses Riboviria Orthornavirae Pisuviricota Pisoniviricetes Sobelivirales Solemoviridae Polerovirus Beet western yellows virus Turnip yellows virus (isolate FL-1) (TuYV) (BWYV-FL1) |
| Enzyme Sequence | MYSKLMFFFALCSISFLFTSEAASTMLLESSYLPLNQSYAPGFLYKRDMLPPPLQAVLTYTCPEPRPLAEESYNDLLRAISQKSSSDFQNAYSLALSFSSDFYQHGLKTLKDVSFLAVEKFLWGLTRLWSSLILASFSALWWLVSNFTTPVFCLALLYTVTKYMVKTVSFLFGGLPIWIISIAFSLLKKSFSALRSTPKCLYEKAIDGFKSFTIPQSPPKSCVIPITHASGNHAGYASCIKLYNGENALMTATHVLRDCPNAVAVSAKGLKTRIPLAEFKTIAKSDKGDVTLLRGPPNWEGLLGCKAANVITAANLAKCKASIYSFDRDGWVSSYAEIVGSEGTDVMVLSHTEGGHSGSPYFNGKTILGVHSGASATGNYNLMAPIPSLPGLTSPTYVFETTAPQGRVFAQEDIAEIEGLYAQVMKRVQQAEDFKPKTGKYWGDMEDDEDIFFESKEDLSGNGVRGTVRGTNGEGSSTPKTSNVDGKEMMEKIISSLVGKINLENIERKVIEEISAKAMKTPKSRRRRAPKKQPESSKDTSPRSTTGKYQPPHVRSPASVTAANCPNTTTPSKKKNLAGGRPSSGTIPRWVRKQAASAGPSSAPKQN |
| Enzyme Length | 607 |
| Uniprot Accession Number | P09506 |
| Absorption | |
| Active Site | ACT_SITE 254; /note=For protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01216; ACT_SITE 289; /note=For protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01216; ACT_SITE 357; /note=For protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01216 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Precursor from which the VPg molecule is probably released at the onset of the RNA synthesis. Essential for virus replication (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (3); Compositional bias (2); Domain (1); Region (2); Signal peptide (1); Site (2); Transmembrane (3) |
| Keywords | Hydrolase;Membrane;Protease;RNA-binding;Reference proteome;Ribosomal frameshifting;Serine protease;Signal;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: [Protein P1]: Membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | PTM: Specific enzymatic cleavages in vivo yield mature proteins. The protease probably cleaves itself and releases the VPg protein (By similarity). {ECO:0000250}. |
| Signal Peptide | SIGNAL 1..22; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 66,211 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |