| IED ID | IndEnz0002007430 |
| Enzyme Type ID | protease007430 |
| Protein Name |
Proteasome subunit pbs-5 EC 3.4.25.1 |
| Gene Name | pbs-5 K05C4.1 |
| Organism | Caenorhabditis elegans |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Nematoda (roundworms) Chromadorea Rhabditida Rhabditina Rhabditomorpha Rhabditoidea Rhabditidae Peloderinae Caenorhabditis Caenorhabditis elegans |
| Enzyme Sequence | MWGETFDDFENDEGEMAMAKQNLIAEPARADFTFAKLPLGIQPVDFMKTHFAETAGKSMQFRKGTTTLAFVYEPATPADKGGIIVAVDSRASSGEYISSKSVMKILDIGDRMVATMAGGAADCQFWTRIVAKYCTLYELREKTSITVSAASKYFANTLYGYRGQGLSVGSMVAGYDKKGPQIFKVDSEGDRCQLKVCSVGSGSLNAYGILDNHYKPKMTDDEARKLGLRAIMHATYRDSGSGGVCNLCHITPTEKIRLPPMDVSKLWYEFADELGRDITYNPVE |
| Enzyme Length | 284 |
| Uniprot Accession Number | Q9XUV0 |
| Absorption | |
| Active Site | ACT_SITE 65; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:P28074 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Cleavage of peptide bonds with very broad specificity.; EC=3.4.25.1; Evidence={ECO:0000250|UniProtKB:P28074}; |
| DNA Binding | |
| EC Number | 3.4.25.1 |
| Enzyme Function | FUNCTION: Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins (PubMed:27528192). This complex plays numerous essential roles within the cell by associating with different regulatory particles (By similarity). Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins (By similarity). The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required (By similarity). {ECO:0000250|UniProtKB:P28074, ECO:0000269|PubMed:27528192}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Propeptide (1) |
| Keywords | Cytoplasm;Hydrolase;Nucleus;Protease;Proteasome;Reference proteome;Threonine protease;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|PROSITE-ProRule:PRU00809}. Nucleus {ECO:0000250|UniProtKB:P28074}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11099033; 11483502; 11559592; 12437114; 12679813; 14551910; 14704431; 15084750; 15221760; 15489339; 15791247; 16122351; 17392428; 21085631; 21177967; 21529718; 21695230; 22560298; 22634595; 23637632; 23800452; 25487147; 26351692; 27506200; 30140741; |
| Motif | |
| Gene Encoded By | |
| Mass | 31,230 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |