| IED ID |
IndEnz0002007499 |
| Enzyme Type ID |
protease007499 |
| Protein Name |
Flotillin-like protein FloA
|
| Gene Name |
floA yqfA BSU25380 |
| Organism |
Bacillus subtilis (strain 168) |
| Taxonomic Lineage |
cellular organisms
Bacteria
Terrabacteria group
Firmicutes
Bacilli
Bacillales
Bacillaceae
Bacillus
Bacillus subtilis group
Bacillus subtilis
Bacillus subtilis subsp. subtilis
Bacillus subtilis (strain 168)
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| Enzyme Sequence |
MDPSTLMILAIVAVAIIVLAVFFTFVPVMLWISALAAGVKISIFTLVGMRLRRVIPNRVVNPLIKAHKAGLNVGTNQLESHYLAGGNVDRVVNALIAAQRANIELTFERCAAIDLAGRDVLEAVQMSVNPKVIETPFIAGVAMDGIEVKAKARITVRANIERLVGGAGEETIVARVGEGIVSTIGSSDNHKKVLENPDMISQTVLGKGLDSGTAFEILSIDIADVDIGKNIGAILQTDQAEADKNIAQAKAEERRAMAVAQEQEMRARVEEMRAKVVEAEAEVPLAMAEALREGNIGVMDYMNIKNIDADTEMRDSFGKLTKDPSDEDRKS |
| Enzyme Length |
331 |
| Uniprot Accession Number |
P54466 |
| Absorption |
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| Active Site |
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| Activity Regulation |
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| Binding Site |
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| Calcium Binding |
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| catalytic Activity |
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| DNA Binding |
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| EC Number |
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| Enzyme Function |
FUNCTION: Found in functional membrane microdomains (FMM) that may be equivalent to eukaryotic membrane rafts. FMMs are highly dynamic and increase in number as cells age. FloA and FloT function is partially redundant; double deletions have marked synthetic phenotypes (PubMed:20713508, PubMed:22753055, PubMed:25909364, PubMed:27362352). Flotillins are thought to be important factors in membrane fluidity, especially during periods of rapid growth in rich media (Probable). Whether specific proteins are associated with FMMs is controversial; in one study FloT rafts have been shown to include proteins involved in adaptation to stationary phase, while FloA-FloT rafts include proteins involved in differentation including sporulation, biofilm formation and DNA uptake competence (PubMed:25909364). Another (more finely resolved) study only showed association of NfeD2 with FloT rafts of all the proteins examined (PubMed:27362352). Involved in spatial organization of membranes, perhaps recruiting proteins to specific membrane regions (PubMed:23651456). Simultaneous overexpression of both FloA and FloT leads to defects in cell division and differentiation, in part caused by stabilization of FtsH and its subsequent increased ability to degrade proteins. Cells make more biofilm, are about half as long, have less EzrA and more frequent Z-rings (PubMed:24222488). {ECO:0000269|PubMed:20713508, ECO:0000269|PubMed:22753055, ECO:0000269|PubMed:23651456, ECO:0000269|PubMed:24222488, ECO:0000269|PubMed:25909364, ECO:0000269|PubMed:27362352, ECO:0000305|PubMed:32662773}. |
| Temperature Dependency |
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| PH Dependency |
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| Pathway |
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| nucleotide Binding |
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| Features |
Chain (1); Motif (4); Mutagenesis (4); Region (2); Transmembrane (2) |
| Keywords |
Cell membrane;Membrane;Reference proteome;Repeat;Transmembrane;Transmembrane helix |
| Interact With |
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| Induction |
INDUCTION: Few foci are seen on rich media, when cells are grown in minimal medium more foci are seen (at protein level) (PubMed:22753055). Constitutively expressed in rich and sporulation/biofilm-inducing media, not controlled by spo0A (at protein level) (PubMed:25909364). {ECO:0000269|PubMed:22753055, ECO:0000269|PubMed:25909364}. |
| Subcellular Location |
SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_01562, ECO:0000269|PubMed:22882210, ECO:0000269|PubMed:23651456, ECO:0000269|PubMed:25909364, ECO:0000269|PubMed:27362352, ECO:0000305|PubMed:20713508, ECO:0000305|PubMed:22753055}; Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_01562}. Membrane raft {ECO:0000255|HAMAP-Rule:MF_01562, ECO:0000269|PubMed:22882210, ECO:0000269|PubMed:25909364, ECO:0000269|PubMed:27362352, ECO:0000305|PubMed:23651456}; Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_01562}. Note=Present in detergent-resistant membrane (DRM) fractions, approximately 6 dynamic foci per cell; sometimes colocalizes with FloT in DRMs (PubMed:20713508, PubMed:22882210, PubMed:23651456). Found in discrete, highly mobile foci, rarely colocalizes with FloT (PubMed:22753055). At the septa colocalizes with FloT and FtsH (PubMed:22882210). Careful analysis gives an average of 13 FloA and 6 FloT foci per cell; FloA foci are smaller that FloT foci (PubMed:25909364). Another study shows FloA and FloT foci are similar in size, forming membrane assemblies of 85-110 nm, but FloA are more mobile than FloT foci. This study found no evidence of colocalization of FloA with FloT (PubMed:27362352). {ECO:0000269|PubMed:20713508, ECO:0000269|PubMed:22753055, ECO:0000269|PubMed:22882210, ECO:0000269|PubMed:23651456, ECO:0000269|PubMed:25909364, ECO:0000269|PubMed:27362352}. |
| Modified Residue |
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| Post Translational Modification |
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| Signal Peptide |
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| Structure 3D |
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| Cross Reference PDB |
- |
| Mapped Pubmed ID |
- |
| Motif |
MOTIF 240..242; /note=EA repeat 1; /evidence=ECO:0000269|PubMed:25909364; MOTIF 251..253; /note=EA repeat 2; /evidence=ECO:0000269|PubMed:25909364; MOTIF 278..282; /note=EA repeat 3; /evidence=ECO:0000269|PubMed:25909364; MOTIF 288..290; /note=EA repeat 4; /evidence=ECO:0000269|PubMed:25909364 |
| Gene Encoded By |
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| Mass |
35,641 |
| Kinetics |
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| Metal Binding |
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| Rhea ID |
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| Cross Reference Brenda |
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