| IED ID |
IndEnz0002007509 |
| Enzyme Type ID |
protease007509 |
| Protein Name |
Serine protease inhibitor Kazal-type 2
|
| Gene Name |
Spink2 |
| Organism |
Mus musculus (Mouse) |
| Taxonomic Lineage |
cellular organisms
Eukaryota
Opisthokonta
Metazoa
Eumetazoa
Bilateria
Deuterostomia
Chordata
Craniata
Vertebrata
Gnathostomata (jawed vertebrates)
Teleostomi
Euteleostomi
Sarcopterygii
Dipnotetrapodomorpha
Tetrapoda
Amniota
Mammalia
Theria
Eutheria
Boreoeutheria
Euarchontoglires
Glires (Rodents and rabbits)
Rodentia
Myomorpha (mice and others)
Muroidea
Muridae
Murinae
Mus
Mus
Mus musculus (Mouse)
|
| Enzyme Sequence |
MLRLVLLLLVTDFAASHETLDSSDSQIMKRSQFRTPDCGHFDFPACPRNLNPVCGTDMNTYSNECTLCMKIREDGSHINIIKDEPC |
| Enzyme Length |
86 |
| Uniprot Accession Number |
Q8BMY7 |
| Absorption |
|
| Active Site |
|
| Activity Regulation |
|
| Binding Site |
|
| Calcium Binding |
|
| catalytic Activity |
|
| DNA Binding |
|
| EC Number |
|
| Enzyme Function |
FUNCTION: As a strong inhibitor of acrosin, it is required for normal spermiogenesis. It probably hinders premature activation of proacrosin and other proteases, thus preventing the cascade of events leading to spermiogenesis defects (PubMed:21705336, PubMed:28554943). May be involved in the regulation of serine protease-dependent germ cell apoptosis (PubMed:21705336). It also inhibits trypsin (PubMed:21705336). {ECO:0000269|PubMed:21705336, ECO:0000269|PubMed:28554943}. |
| Temperature Dependency |
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| PH Dependency |
|
| Pathway |
|
| nucleotide Binding |
|
| Features |
Alternative sequence (1); Chain (1); Disulfide bond (3); Domain (1); Signal peptide (1); Site (1) |
| Keywords |
Alternative initiation;Apoptosis;Cytoplasmic vesicle;Differentiation;Direct protein sequencing;Disulfide bond;Protease inhibitor;Reference proteome;Secreted;Serine protease inhibitor;Signal;Spermatogenesis |
| Interact With |
|
| Induction |
|
| Subcellular Location |
SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P20155}. Cytoplasmic vesicle, secretory vesicle, acrosome {ECO:0000269|PubMed:28554943}. |
| Modified Residue |
|
| Post Translational Modification |
|
| Signal Peptide |
SIGNAL 1..16; /evidence=ECO:0000255 |
| Structure 3D |
|
| Cross Reference PDB |
- |
| Mapped Pubmed ID |
11217851;
12466851;
14610273;
21267068;
24194600;
24699552;
27626380;
31146259;
|
| Motif |
|
| Gene Encoded By |
|
| Mass |
9,722 |
| Kinetics |
|
| Metal Binding |
|
| Rhea ID |
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| Cross Reference Brenda |
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